Comparison of Monoclonal Antibody Based Serological Tests in the Detection of Infectious Bursal Disease Virus
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Date
2005
Journal Title
Journal ISSN
Volume Title
Publisher
Royal Veterinary Journal of India
Abstract
Monoclonal antibody based antigen capture ELISA (AC-ELISA) and Dot-ELISA were carried out for the detection of infectious bursal disease virus (IBDV) and compared with agar gel immunodiffusion (AGID) test to study their suitability for routine laboratory. For this study, bursal samples from 120 poultry flocks, suspected for infectious bursal disease (IBD) were screened by AC-ELISA, Dot-ELISA and agar gel immunodiffusion (AGID) test using monoclonal antibodies. These three tests were compared for their efficiency in the detection of IBD. Out of 120 samples tested, AC-ELISA detected 74 (62%) as positive, Dot-ELISA detected 70 (58%) and AGID test detected only 53 (40%) as positive for IBDV antigen. AC-ELISA was found to be more sensitive followed by Dot-ELISA than AGID test in the detection of IBDV antigen from field specimens.
Description
Keywords
Infectious Bursal Disease, Serological Tests, Monoclonal antibodies