Molecular, Morphological and Chemical Characterization of Tomato (Solanum lycopersicum L.) Genotypes and Influence of Different Organic Manures on Seed Yield, Quality in Tomato Cv. DMT-2
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Date
2011
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UAS, Dharwad
Abstract
The laboratory and field experiments were conducted, during kharif, 2010 for
identification of tomato genotypes through morphological, chemical and molecular
markers and influence of organic manures on seed yield and seed quality in tomato
cv. DMT-2 at Seed Quality and Research Laboratory of National Seed Project, and
main agricultural research station UAS, Dharwad respectively.
Twelve tomato genotypes were grouped into 18 groups based on the seed
morphological characters such as seedling leaf color, days to maturity, foliage density,
leaf type, exterior color of immature fruit, fruit shape fruit size, exterior color of
mature fruit, from pedicel, fruit shoulder shape, skin color ripe fruit, flesh color
intensity, fruit cross sectional shape, number of locules, shape pistil scar and fruit
blossom end shape.
The chemical tests viz., Phenol, modified phenol, Peroxidase, NaOH and KOH
tests disabled the grouping of tomato genotypes based on the color response.
Genotypes used in this investigation did not exhibit change in seed coat color as well
as solution. Based on the seedling growth response to GA3, genotypes were grouped
as low, moderate and high response and based on 2,4-D genotypes were grouped as
least, moderate and highly affected.
Random amplified polymorphic DNA profile for all 12 genotypes was
generated with 20 random decamer primers. The highest molecular diversity was
observed between the genotypes DMT-3 and DMT-6 and High similarity with Sij
0.99 was observed between genotypes DMT-5 and DM-4.
The field experiment consisted of 11 treatments involving different organic
manures. Among the treatments recommended dose of fertilizer (T11) recorded
significantly superior values over other treatments with respect to growth, yield and
seed quality parameters.
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Seed Science and Technology