Effect of different plant growth regulators, sugars and gelling agents on in-vitro multiplication and different potting mixtures on survival rate of in-vitro raised plants of Brahmi [Bacopa monnieri (L.)]
dc.contributor.advisor | Dahiya, G.S. | |
dc.contributor.author | Manish Kumar | |
dc.date.accessioned | 2023-07-18T04:17:03Z | |
dc.date.available | 2023-07-18T04:17:03Z | |
dc.date.issued | 2022-07 | |
dc.description.abstract | The investigation was carried out to study the effect of different plant growth regulators, sugars and gelling agents on in-vitro multiplication and different potting mixtures on survival rate of in-vitro raised plants of Brahmi [Bacopa monnieri (L.)]. For in-vitro multiplication plant cultures were inoculated on MS media supplemented with different plant growth regulators (BAP/Kn and IAA/NAA), carbon sources (fructose, sucrose, glucose, table sugar and maltose), gelling agens (agar-agar, agarose, gelrite, guargum and isabgol). The maximum shoots were observed on MS medium supplemented with M2 (4.6±0.67), M12 (5.0±0.04), M32 (4.9±0.06) and M19 (5.0±0.19). The maximum number of shoots (11.8±0.22) observed when MS media is supplemented with table sugar. The very good growth/rooting was observed on ½ basal media. The well rooting plantlets were tansplanted into pots containing various components of soil, sand, vermicompost, FYM, coco pit and perlite. The maximum (100%) survival were observed in four different mix including sand : soil : FYM, sand : soil: vermicompost, sand : soil : FYM : vermicompost and coco pit : vermicompost : perlite under green house conditions. Hardened plants were successfully transferred to the field conditions. | en_US |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810198316 | |
dc.keywords | MS media, Tissue cuture, Brahmi, in-vitro, Carbon sources, Gelling agents | en_US |
dc.language.iso | English | en_US |
dc.pages | 40 + iv | en_US |
dc.publisher | CCS HAU, Hisar | en_US |
dc.sub | Genetics and Plant Breeding | en_US |
dc.theme | Effect of different plant growth regulators, sugars and gelling agents on in-vitro multiplication and different potting mixtures on survival rate of in-vitro raised plants of Brahmi [Bacopa monnieri (L.)] | en_US |
dc.these.type | M.Sc | en_US |
dc.title | Effect of different plant growth regulators, sugars and gelling agents on in-vitro multiplication and different potting mixtures on survival rate of in-vitro raised plants of Brahmi [Bacopa monnieri (L.)] | en_US |
dc.type | Thesis | en_US |