ISOLATION AND EVALUATION OF ENDOPHYTES FROM GRAIN AMARANTHUS SPECIES FOR PLANT GROWTH PROMOTING ACTIVITIES
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Date
2018-08-01
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University of Agricultural Sciences, Bangalore
Abstract
A study was undertaken to isolate and evaluate endophytes from grain Amaranthus species for plant growth promoting activities. Endophytic bacteria are found in association with plants. In this study, twenty bacteria were isolated from root, shoot and leaves of two varieties of grain amaranth, Suvarna and KBGH-1. Out of which, most of them were Gram positive and have shown positive results for N-fixation, Psolubilisation, growth hormone production, siderophore production and biocontrol activity against plant pathogens under laboratory condition. They were efficient in controlling major soilborne pathogens of grain amaranth through production of siderophore and by possessing ACC deaminase activity. Among twenty isolates, five efficient bacterial isolates namely RAR-5, RAS-3, RAL-7, WAR-1 and WAS-11 were
selected to test for their efficiency of influencing plant growth and biocontrol activity against Pythium aphanidermatum, Rhizoctonia solani and Fusarium oxysporum in Suvarna and KBGH-1 varieties of grain amaranth under glass house condition. From the results obtained, it was found that the selected isolates were able to control the nursery diseases in effective manner. These efficient isolates were subjected to 16S rRNA gene sequencing and were identified as Alcaligenes faecalis subsp. phenolicus, Pseudomonas moorei, Bacillus siamensis, Bacillus cereus and Acromobacter deleyi. These bacterial
endophytes either individually or in combination with reduced amount of fertilizers improved plant growth and yield of the grain amaranth. These results strongly suggest that the endophytic bacteria characterized in this study could be successfully used to promote plant growth and inducing fungal resistance in plant.