ROLE OF NARINGENIN ON THE ANTIBACTERIAL ACTIVITY OF SELECTED β-LACTAM ANTIBIOTICS
| dc.contributor.advisor | SRIVIDYA, G (MAJOR) | |
| dc.contributor.advisor | RAVI KUMAR, P | |
| dc.contributor.advisor | SUDHAKAR, K | |
| dc.contributor.author | RAJENDRA CHOWDARY, M | |
| dc.date.accessioned | 2022-09-22T13:39:41Z | |
| dc.date.available | 2022-09-22T13:39:41Z | |
| dc.date.issued | 2022-03 | |
| dc.description | THESES | en_US |
| dc.description.abstract | Beta-lactam antibiotics are one of the most commonly used antibacterial agents against different bacterial infections in livestock. Among them ampicillin and amoxicillin are most widely used by the clinicians in both human and veterinary practice. However, their indiscriminate usage resulted in their reduced efficacy and development of resistance by microorganisms. To combat the resistance and restore the antibacterial efficacy of the antibiotics, their combination with non antibiotic drugs like phytochemicals is being considered actively.In the present study the drug interaction between selected β-lactam antibiotics (ampicillin and amoxicillin) and phytochemical naringenin was evaluated against Escherichia coli and Staphylococcus aureus invitro using micro broth dilution method, crystal violet assay and checker board method. MIC, MBC, FIC, and biofilm inhibition values were used as indicators to determine the antibacterial activity. MIC values of ampicillin, amoxicillin and naringenin against Escherichia coli were 6.25μg/ml, 12.5μg/ml and 2.5mg/ml respectively. The minimum inhibitory concentration values of ampicillin, amoxicillin and naringenin against Staphylococcus aureus were 0.312μg/ml and 0.312μg/ml and 0.625mg/ml respectively. The MIC values of ampicillin and amoxicillin were reduced by 2-fold in the presence of naringenin. The MBC values of ampicillin were significantly reduced from 25μg/ml to 12.5μg/ml in presence of naringenin against Escherichia coli and from 1.25μg/ml to 0.625 μg/ml against Staphylococcus aureus. FIC value of combination of ampicillin with naringenin against Escherichia coli and Staphylococcus aureus was 1 indicating the additive effect of the combination. The MBC values of amoxicillin were significantly reduced from 50μg/ml to 25μg/ml in presence of naringenin against Escherichia coli and from 1.25μg/ml to 0.625 μg/ml against Staphylococcus aureus. FIC value of combination of amoxicillin with naringenin against Escherichia coli and Staphylococcus aureus was 1 indicating the additive effect of the combination. Ampicillin, amoxicillin and naringenin alone inhibited the Escherichia coli biofilm formation by 63.5±2.25, 65.1±2.32 and 46.6±5.32 % respectively and combination of ampicillin with naringenin and amoxicillin with naringenin inhibited the biofilm formation by 44.3±2.89 and 54.4±2.68 % respectively. xvi Ampicillin, amoxicillin and naringenin alone inhibited the Staphylococcus aureus biofilm formation by 59.3±2.28, 59.7±2.41 and 33.02±6.8 % respectively and combination of ampicillin with naringenin and amoxicillin with naringenin inhibited the biofilm formation by 32.65±3.49 and 45.9±2.31% respectively. Based on the above results, it can be concluded that naringenin produced positive drug interaction with ampicillin and amoxicillin by reduction in the MIC and MBC values significantly. Further, in-vivo studies are required to establish antibacterial mechanism and biofilm inhibition mechanisms of naringenin to develop it into a therapeutic agent against infectious diseases. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810188049 | |
| dc.keywords | NARINGENIN; ANTIBACTERIAL ACTIVITY; β-LACTAM ANTIBIOTICS; ROLE | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 90 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA | en_US |
| dc.sub | Veterinary Pharmacology and Toxicology | en_US |
| dc.theme | ROLE OF NARINGENIN ON THE ANTIBACTERIAL ACTIVITY OF SELECTED β-LACTAM ANTIBIOTICS | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | ROLE OF NARINGENIN ON THE ANTIBACTERIAL ACTIVITY OF SELECTED β-LACTAM ANTIBIOTICS | en_US |
| dc.type | Thesis | en_US |