Selection and characterization of chickpea calli tolerant to ascochyta rabiei (Pass.) Labrousse

Simple item page
dc.contributor.advisorSangwan, M.S.
dc.contributor.authorPrabhat Kumar
dc.date.accessioned2016-11-16T14:11:12Z
dc.date.available2016-11-16T14:11:12Z
dc.date.issued2009
dc.description.abstractPresent study was carried out with the objective to develop efficient protocol for establishment of callus culture derived from hypocotyl explant, to evaluate the callogenic response of chickpea genotypes against different concentrations of culture filtrate of Ascochyta rabiei and biochemical characterization of calli showing resistance to culture filtrate. Among different media used for callus induction, MS medium supplemented with IAA (2.5 mg/l) & BAP (0.5 mg/l) [C4] gave maximum callus induction (100 %), also dark green and fragile callus. Callogenic development was more in resistant [E100Y(m)] and moderately resistant (Gaurav) genotypes as compared to susceptible genotypes Pb-7 and L550, when exposed to different concentrations of A. rabiei culture filtrate. Addition of 20 % concentration of culture filtrate in callus induction medium proved to be lethal for most of chickpea genotypes calli. Therefore, sub lethal concentrations up to of 15 % can be used for in vitro screening for resistant calli. Increase in concentrations of culture filtrate resulted in decrease of fresh as well as dry weight of calli derived from all genotypes. Total soluble sugars and reducing sugars were higher in calli of resistant [E100y (m)] and moderately resistant (Gaurav) genotypes as compared to susceptible genotypes (Pb-7, L 550). Level of phenols, flavanols and soluble proteins were also higher in the calli of all genotypes with increased in concentrations of culture filtrate. Activities of all the three enzymes viz. polyhenol oxidase, peroxidase and catalase increased in response to increase in concentrations of culture filtrate. But activities of polyphenol oxidase and peroxidase were higher in moderately resistant and resistant genotypes while catalase activity was higher in susceptible genotypes.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/85858
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subPlant Pathology
dc.subjectChickpea, Hypocotyl, Callus, Culture filtrate, Ascochyta rabiei, Resistance, Biochemicalen_US
dc.these.typeM.Sc
dc.titleSelection and characterization of chickpea calli tolerant to ascochyta rabiei (Pass.) Labrousseen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
CCSHAU-291742-Prabhat Kumar.pdf
Size:
7.46 MB
Format:
Adobe Portable Document Format
Description:
Selection and characterization of chickpea calli tolerant to ascochyta rabiei (Pass.) Labrousse
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.28 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
M. Sc. Dissertations