Selection and characterization of chickpea calli tolerant to ascochyta rabiei (Pass.) Labrousse
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Date
2009
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Publisher
CCSHAU
Abstract
Present study was carried out with the objective to develop
efficient protocol for establishment of callus culture derived from hypocotyl
explant, to evaluate the callogenic response of chickpea genotypes against
different concentrations of culture filtrate of Ascochyta rabiei and biochemical
characterization of calli showing resistance to culture filtrate. Among different
media used for callus induction, MS medium supplemented with IAA (2.5
mg/l) & BAP (0.5 mg/l) [C4] gave maximum callus induction (100 %), also
dark green and fragile callus. Callogenic development was more in resistant
[E100Y(m)] and moderately resistant (Gaurav) genotypes as compared to
susceptible genotypes Pb-7 and L550, when exposed to different
concentrations of A. rabiei culture filtrate. Addition of 20 % concentration of
culture filtrate in callus induction medium proved to be lethal for most of
chickpea genotypes calli. Therefore, sub lethal concentrations up to of 15 %
can be used for in vitro screening for resistant calli. Increase in
concentrations of culture filtrate resulted in decrease of fresh as well as dry
weight of calli derived from all genotypes. Total soluble sugars and reducing
sugars were higher in calli of resistant [E100y (m)] and moderately resistant
(Gaurav) genotypes as compared to susceptible genotypes (Pb-7, L 550). Level
of phenols, flavanols and soluble proteins were also higher in the calli of all
genotypes with increased in concentrations of culture filtrate. Activities of all
the three enzymes viz. polyhenol oxidase, peroxidase and catalase increased
in response to increase in concentrations of culture filtrate. But activities of
polyphenol oxidase and peroxidase were higher in moderately resistant and
resistant genotypes while catalase activity was higher in susceptible
genotypes.
Description
Keywords
Chickpea, Hypocotyl, Callus, Culture filtrate, Ascochyta rabiei, Resistance, Biochemical