Molecular and biochemical screening of rice genotypes against salinity and drought (dual) stresses
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Date
2021-04-16
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ANDUAT, Kumarganj, Ayodhya
Abstract
The present investigation entitled “Molecular and biochemical screening of
rice genotypes against salinity and drought (dual) stresses” was under taken for
screening of twenty four rice genotypes namely, Taramon, NEDU, IR91167-31-3-1-
33, IR91167-99-1-1-1-3, IR91171-66-3-2-1-3, IR92953-49-1-3, NUD3, NDRK5088,
NUD2, NDR359, CSR30, IR 29, IR64, CSR13, IR28, SARJOO52, AYAAR,
AMKER, FL478, NDRK2008, Nageena 22, NDR 1, Baranideep and Sushk Samrat.
Objectives were to evaluate rice varieties for salt and drought stresses (dual stress) at
seedling and reproductive stages, to identify dual tolerant rice genotypes and to
pyramid salt and drought tolerant gene(s)/QTL(s) in elite rice genotype. Screening of
rice genotypes at seedling stage was carried out in hydroponics system in lab
condition Twenty four rice genotypes after 21 days salinization tolerant genotypes
were CSR 30 and CSR 13. The susceptible score 7 exhibited by NDR359, IR29 and
IR 28. For drought stress screening at vegetative and reproductive stage observations
were recorded for seventeen plant characters viz., seedling vigour (cm), days to 50%
flowering, plant height (cm), panicle bearing tillers per plant, panicle length number
of spikelets per panicle, number of grains per panicle, spikelet fertility (%), test
weight (g), panicle bearing tillers per plant, biological yield per plant (g), harvest
index (%), relative water content (RWC), leaf rolling, drought recovery and grain
yield per plant and biochemical screening for drought and salinity with measurement
of the chlorophyll content, proline content, sodium oxide dismutase (SOD),
Peroxidase, Catalase and total soluble sugar content were recorded. The analysis of
variance involving 24 rice genotypes with regards to yield, yield components and
quality traits was done for seventeen characters under drought and thirteen characters
under normal conditions. The mean sum of square values for interaction effects of
traits among 24 rice genotypes under drought treatment were found significant for
almost all the traits except panicle bearing tillers and leaf rolling at reproductive stage.
The imposed drought comparatively has impacted negatively and causes reduction in
almost all studied traits comparatively to normal condition.
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