COMPARISON OF THE DIAGNOSTIC EFFICACIES OF RECOMBINANT LIPL21 AND LIPL32 PROTEINS IN CANINE LEPTOSPIROSIS
Loading...
Date
2015
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR
Abstract
Leptospirosis is a highly endemic disease in Kerala affecting man and
animals. Microscopic Agglutination Test (MAT), though, the gold standard test for
leptospirosis is often a less preferred choice since it is very cumbersome and serovar
specific. Alternatively, genus specific tests like ELISA are considered more
appropriate for routine diagnosis of leptospirosis. In the present study, recombinant
outer membrane proteins of Leptospira,viz. rLipL21 and rLipL32 were used as
antigens in ELISA for detection of IgG specific to Leptospira. The corresponding
genesie., lipl21 and lipl32 were cloned,expressed and purified to produce
recombinant proteins. Sera samples collected from 124 suspected cases of
leptospirosis were subjected to MAT and were subsequently tested in ELISAs. The
sensitivity and specificity of rLipL21 ELISA was found to be 90.90% and 73.52%,
respectively. Similarly for rLipL32 ELISA the values were 81.81% and78.43%,
respectively. On statistical analysis it was observed that both the ELISAs were
promising tests for the diagnosis of leptospirosis as compared to MAT. Also there
observed no significant differences in the diagnostic efficacies of the two ELISAs
ensuring their potential as genus specific diagnostics
Description
Keywords
null