Epidemiological investigation and therapeutic evaluation of azadirachta indica in bovine subclinical mastitis
Loading...
Date
2015-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
During present study crossbred cows maintained at I.D.F., G.B. Pant University of Agriculture and Technology, Pantnagar, were screened using California Mastitis Test. The prevalence was recorded cow wise, quarter wise, age wise, parity wise, lactation stage wise, lactation yield wise and quarter infection rate wise. In vitro antimicrobials susceptibility pattern of bacterial isolates was determined and therapeutic efficacy of methanolic and hydromethanolic extracts of Azadirachta indica leaves were evaluated against bovine subclinical mastitis. The prevalence of subclinical mastitis (SCM) was recorded as cow (56.25%) and quarter (33.17%) wise with higher involvement of right hindquarters. The prevalence was highest (19.04%) in third parity and early stage (41.26%) of lactation. Microbiological examination revealed maximum involvement of Staphylococcus spp. (66.90%), followed by Streptococcus spp. (23.23%), E.coli. (02.81%) and mixed infection (07.04%) in SCM cows. During the study, antibiogram pattern revealed that in comparison to other antibiotics, enrofloxacin and cefotaxime were highly sensitive against Staphylococcus spp., Streptococcus spp. and E.coli. The cows of group III, IV and V showed significant decrease of CMT point score, pH value, TBC and SCC on day 15 and 30 in comparison to day 0. Milk fat, protein and lactose did not revealed any significant alteration on day 30 as compared to day 0 while milk SNF and total solids increased significantly on day 30 in comparison to day 0 in both the extract treated groups (IV and V). The CMT score, pH value, SCC, neutrophil and lymphocyte percentage in groups IV and V were significantly higher on day 3 post treatment in comparison to group III which were comparable on day 7 and were normal by day 30 post treatment. The highest recovery 83.33% was recorded in SCM affected cows in group III followed by 66.66% in group IV and 50.00% in group IV. No adverse reaction and milk losses were observed in A. indica extracts treated groups during the period of treatment. On the basis of results of present investigation, the use of methanolic extract of A. indica may be recommended as an alternative to the antibiotic treatment for management of subclinical mastitis in cows.
Description
Keywords
null