Demonstration of Alternative and Classical Complement Pathway Activity in Colostrum from Buffalo (Bubalus Bubalis)

Simple item page
dc.contributor.authorMatheswaran, K.
dc.contributor.authorRaj, G. Dhinakar
dc.contributor.authorNachimuthu, K.
dc.contributor.authorTANUVAS
dc.date.accessioned2016-08-10T14:12:21Z
dc.date.available2016-08-10T14:12:21Z
dc.date.issued2003
dc.description.abstractBuffalo colostrums caused lysis of unsensitized red blood cells (RBC) from sheep, goats, rabbits and chickens. RBC from cattle and buffalo were resistant to lysis. That lysis was due to the presence of natural antibodies to these RBC was ruled out since there was no reduction in haemolytic titres even after adsorption with the respective RBC. The addition of EGTA to the diluents had no effect on the haemolytic activity. These findings indicate the presence of alternative complement pathway (ACP) activity in buffalo colostrums. The haemoloytic activity of buffalo complement for unsensitized rabbit RBC was reduced to very low levels by heating at 50°C for 45 min. Treatment with zymosan also inhibited the haemolytic activity, while inulin had no effect. The maximum activity of ACP occurred in the presence of 4 mmol/L Mg^2+ in the diluent. The range of ACP activities in colostrums fro buffaloes varied from 4.06 to 8.48 CH50 units/ml. Using a standard system for titrating the classical complement pathway and rabbit red blood cells sensitized with goat haemolysin, the range of complement activity in buffalo colostrums was 4.81-6.77 CH50/ml.
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/71927
dc.language.isoenen_US
dc.publisherVeterinary Research Communicationsen_US
dc.subjectAlternate Pathwayen_US
dc.subjectBuffaloen_US
dc.subjectClassical Pathwayen_US
dc.subjectColostrumen_US
dc.subjectComplementen_US
dc.subjectHeat Treatmenten_US
dc.subjectHaemolysinen_US
dc.subjectInulinen_US
dc.subjectMagnesiumen_US
dc.subjectZymosanen_US
dc.titleDemonstration of Alternative and Classical Complement Pathway Activity in Colostrum from Buffalo (Bubalus Bubalis)en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
GDR_50.pdf
Size:
264.65 KB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.28 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Articles (1.Journal)