IN VITRO ASSESSMENT OF VASICINE AS AN AMELIORATOR OF MYCOTOXINS (AFLATOXIN B1 AND OCHRATOXIN A ) IN CELL LINE
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Date
2021
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Abstract
Mycotoxin contamination of agricultural crops produce huge economic loss each year. Contamination of animal feed also causes damage by reducing the productivity of animals and adversely affecting animal and human health. AFB1, OTA and CTN are three important mycotoxins that are of major concern pertaining to safety and economic issues. Pre and post harvest methods are adopted to control the issue of mycotoxicosis , however pre harvest methods like applications of fungicides come with a set of limitations. Post harvest practices are mainly classified to physical, chemical and biological methods. But these too show numerous disadvantages like nutrient loss, harmful effects produced by chemicals etc. Use of phytochemicals for detoxification as well as mitigation of the adverse effects of mycotoxins are a suitable and emerging alternative in this scenario.
Vasicine is the major alkaloid present in the perennial shrub Adhatoda vasica, which has documented anti oxidant and anti inflammatory properties. The remedial potential of the phytochemical in mycotoxins was explored in the study. In the present study, vasicine was extracted from the leaves of A. vasica by traditional acid-base extraction procedure and subsequently was purified by preparative TLC. Vasicine was characterized by FTIR and quantified by UV-Vis spectrophotometry. For the in vitro studies, suitable concentrations were chosen as 1.25, 2.5 and 5 μg/ml, such that more than 90% cell viability was observed in BHK 21 cell line by MTT assay. The effect of vasicine against mycotoxins was compared to the action of silymarin at 10 μg/ml.combating the noxious effect of