Mass multiplication of Clonostachys rosea and its pathogenicity against insect pest
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Date
2020-11
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
A study regarding collection, isolation, identification pathogenicity, mass multiplication and compatibility with botanical extracts was conducted for Clonostachys rosea. This entomopathogen was isolated from G. B. P. U. A & T Pantnagar, Udhamsingh Nagar, Uttrakhand; during 2018-2020. Results regarding the mass multiplication of Clonostachys rosea depicted that best bio-substrate is Maize grains+ Yeast+ Dextrose with highest biomass on 14 days (38.07 gm) and highest conidial count and germination percent on 21 days (3.33× 104/ml and 79.13%). In the pathogenicity test against Mango hopper (Amritodus atkinsoni), Whitefly (Bemisia tabaci), Okra jassids (Amrasca sp.), Aphids (Lipaphis erysimi) and Tobacco caterpillar (Spodoptera litura); it was found that the highest mean mortality was obtained for Mango Hoppers (93.33%) at a concentration of 4× 109 conidia/ml, for whitefly, the highest mortality documented was 70.00% at a concentration of 3.5× 108 conidia/ml and for okra jassids, the highest mortality observed was 80.00% at a concentration of 3.5× 108 conidia/ml . No mortality was observed for Aphid and Tobacco caterpillar. In the compatibilty test of Clonostachys rosea with extracts of Marigold, Periwinkle, Custard apple and Crysanthemum; it was observed that Marigold extract was highly incompatible at concentration of 75% with mean radial growth of 0.87 cm (reduction over control of 81.48%) and mean conidial count of 0.25× 104/ml (reduction over control of 90.91%). While Periwinkle extract was found to be least toxic to C. rosea. The highest concentration of 75% resulted in mean radial growth of 3.17cm (reduction over control of 32.55%) and mean conidial count of 1.50× 104/ml (reduction over control of 45.45%). Thus from the study it was concluded that the fungus is potential in controlling insect pest and can be included in IPM module. And further studies should be conducted to check the bioefficacy of the fungus against other major insect pest prevalent in the ecosystem.