Yellow mosaic disease resistance in Vigna species and potential green bridges for legumoviruses in Punjab

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dc.contributor.advisorSirari, Asmita
dc.contributor.authorRohan Kumar
dc.date.accessioned2022-02-24T10:36:25Z
dc.date.available2022-02-24T10:36:25Z
dc.date.issued2021
dc.description.abstractYellow Mosaic Disease (YMD) is reported to be the most destructive viral disease on mungbean caused by Yellow mosaic Virus belonging to genus Begomovirus of the family Geminiviridae. Development of YMD resistance in cultivated mungbean varieties remained unsuccessful due to highly variable nature of pathogen. In present study, 12 wild accession of mungbean and various weeds were collected. In different wild accession level of resistance were analysed in natural field condition and in artificial condition, samples were subjected to specific primers of LYMV (MYMV/MYMIV) out of 12 wild accession 4 accession were showing HR (high resistance) Vigna umbellata (GP1), V. radiata var satulosa (GP11) V. sublobata (GP2) and V. sublobata (GP12). All accessions showed presence of whitefly but count on HR accession (Vigna umbellata (GP1)(11.66), V. radiata var satulosa (GP11)(9.33), V. sublobata (GP2)(10.00) and V. sublobata (GP12)(12.13)) was less than susceptible check(SML 1082)(23.66). 36 weeds were collected from different locations of Punjab and were subjected to RCA and out of 36 samples 9 samples were amplified specific band size 2.8kb afterward these sample subjected to LYMV specific primer (MYMV/MYMIV). All 9 samples showed presence of MYMIV virus but MYMV virus was not present in any sample. Then to know the presence other virus in these 9 samples. Keeping this in view, in present study, the degenerate primers PALIc1960 and PARIv72 were used to amplify the RCA enriched DNA of weeds. All nine weeds showed association of Begomoviruses, confirmed with amplicon of ~1.2bp. Further, these amplicons were sequenced for characterization of Begomoviruses harbouring in weeds during off season by outsourcing from Agri Genome Pvt. Ltd. The nucleotide-BLAST results of sequences revealed the presence of Tomato leaf curl Palampur virus in Baru (Sorghum halepense), Ivy gourd, (Coccinia grandis), Leh, (Cirsium arvense), Button weed, (Malva parviflora), Jangli halon (Coronopus didymus), Maina (Medicago polymorpha) and Tik weed (Tridax procubens), while Billygoat weed (Ageratum conyzoides) and Sadabahar (Catharanthus roseus) showed presence of Ageratum enation virus and Papaya leaf curl virus respectively.en_US
dc.identifier.citationRohan Kumar (2021). Yellow mosaic disease resistance in Vigna species and potential green bridges for legumoviruses in Punjab (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810182827
dc.keywordsYMD, YMV, Resistance, Weeds, RCA (rolling circle amplification), MYMVen_US
dc.language.isoEnglishen_US
dc.pages63en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemYellow mosaic disease resistance in Vigna species and potential green bridges for legumoviruses in Punjaben_US
dc.subPlant Pathologyen_US
dc.themeYellow mosaic disease resistance in Vigna species and potential green bridges for legumoviruses in Punjaben_US
dc.these.typeM.Scen_US
dc.titleYellow mosaic disease resistance in Vigna species and potential green bridges for legumoviruses in Punjaben_US
dc.typeThesisen_US
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