Yellow mosaic disease resistance in Vigna species and potential green bridges for legumoviruses in Punjab
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
Yellow Mosaic Disease (YMD) is reported to be the most destructive viral disease on
mungbean caused by Yellow mosaic Virus belonging to genus Begomovirus of the family
Geminiviridae. Development of YMD resistance in cultivated mungbean varieties remained
unsuccessful due to highly variable nature of pathogen. In present study, 12 wild accession of
mungbean and various weeds were collected. In different wild accession level of resistance
were analysed in natural field condition and in artificial condition, samples were subjected to
specific primers of LYMV (MYMV/MYMIV) out of 12 wild accession 4 accession were
showing HR (high resistance) Vigna umbellata (GP1), V. radiata var satulosa (GP11) V.
sublobata (GP2) and V. sublobata (GP12). All accessions showed presence of whitefly but
count on HR accession (Vigna umbellata (GP1)(11.66), V. radiata var satulosa (GP11)(9.33),
V. sublobata (GP2)(10.00) and V. sublobata (GP12)(12.13)) was less than susceptible
check(SML 1082)(23.66). 36 weeds were collected from different locations of Punjab and
were subjected to RCA and out of 36 samples 9 samples were amplified specific band size
2.8kb afterward these sample subjected to LYMV specific primer (MYMV/MYMIV). All 9
samples showed presence of MYMIV virus but MYMV virus was not present in any sample.
Then to know the presence other virus in these 9 samples. Keeping this in view, in present
study, the degenerate primers PALIc1960 and PARIv72 were used to amplify the RCA
enriched DNA of weeds. All nine weeds showed association of Begomoviruses, confirmed
with amplicon of ~1.2bp. Further, these amplicons were sequenced for characterization of
Begomoviruses harbouring in weeds during off season by outsourcing from Agri Genome
Pvt. Ltd. The nucleotide-BLAST results of sequences revealed the presence of Tomato leaf
curl Palampur virus in Baru (Sorghum halepense), Ivy gourd, (Coccinia grandis), Leh,
(Cirsium arvense), Button weed, (Malva parviflora), Jangli halon (Coronopus didymus),
Maina (Medicago polymorpha) and Tik weed (Tridax procubens), while Billygoat weed
(Ageratum conyzoides) and Sadabahar (Catharanthus roseus) showed presence of Ageratum
enation virus and Papaya leaf curl virus respectively.
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Citation
Rohan Kumar (2021). Yellow mosaic disease resistance in Vigna species and potential green bridges for legumoviruses in Punjab (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.