Studies on osmoprotective role of trehalose in thermotolerance of wheat (triticum aestivum L.)
| dc.contributor.advisor | Madan, Shashi | |
| dc.contributor.author | Yogita | |
| dc.date.accessioned | 2017-06-03T03:20:55Z | |
| dc.date.available | 2017-06-03T03:20:55Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | The present investigation was carried out to study the osmoprotective role of trehalose in thermotolerance of wheat (Triticum aestivum L.). Two wheat genotypes viz. WH-730 (thermotolerant) and WH-711 (thermo-sensitive) were grown under control conditions in seed germinator at 25ºC. The 10 days old seedlings were exposed to 35ºC. These seedlings were supplied with Hogland solution containing 1mM and 2mM trehalose. All biochemical (enzymatic and non-enzymatic) and physiological parameters were studied in the leaves of control (25ºC), stressed (35ºC) and trehalose (1mM and 2mM) treated seedlings on 15th day of germination. Analysis of data revealed that activity of H2O2 scavenging enzymes such as CAT, POX and APX increased under heat stress in both the genotypes while decreased with the increasing concentration of trehalose from 1mM to 2mM. The LOX activity, MDA and H2O2 content increased under heat stress conditions when compared with controls reflecting the higher lipid peroxidation and free radical production under stressed conditions whereas trehalose helped to recover from damage due to lipid peroxidation and free radical production. The metabolites like ascorbic acid and trehalose were increased under stressed conditions. Upon trehalose treatment, reduction in ascorbic acid content was observed while trehalose content further increased under trehalose treatment. The glucose content reduced under heat stress, but increased in treated seedlings. Physiological parameters like relative water content, osmotic potential, membrane thermo-stability and ratio of chlorophyll a and b were found to be higher in trehalose treated seedlings in comparison to stressed conditions. Excised leaf water loss capacity (ELWL) increased under stress conditions whereas trehalose treatment decreased ELWL. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810014003 | |
| dc.language.iso | en | en_US |
| dc.publisher | CCSHAU | en_US |
| dc.sub | Biochemistry | |
| dc.subject | Wheat, Trehalose, Physiological, Biochemical, Heat stress | en_US |
| dc.these.type | Ph.D | |
| dc.title | Studies on osmoprotective role of trehalose in thermotolerance of wheat (triticum aestivum L.) | en_US |
| dc.type | Thesis | en_US |
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