Cytokine response to killed Staphylococcus pseudintermedius antigen in dogs with skin diseases
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Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Agricultural Research Communication Centre
Abstract
The attempt was made to study the response of the dog having skin infection with various underlying causes to killed
S.pseudintermedius antigen. PBMC were separated and sensitized with killed S. pseudintermedius for 24 hours. cDNA
was made from extracted RNA and quantitative PCR were carried out for 8 cytokines with GAPDH as housekeeping gene.
IL-6 was the cytokine which was expressed in a statistically significant high level in PBMC of healthy animals to killed
antigen than PBMC of infected animals. Except for the IL-6, all other cytokines were expressed at high level in pyoderma
animals than healthy control, demodicosis and allergy cases. In the present study, IL-1β, IL-8 and TNF-α were the cytokines
that were up-regulated and, IL-6 and IFN-γ were down-regulated in demodicosis dogs with pyoderma at apparently significant
level than the other tested cytokines. IL-4 was the only cytokine that was expressed in measurable quantity in allergic cases
when compared to other cytokines. Thus, it was concluded that dogs with staphylococcal pyoderma infections developed
a Th1/Th2 response to fight the infection.
Description
TNV_IJAR_2018_1-5
Keywords
Veterinary Science