CONCORDANCE OF POLLEN AND PLANT RESPONSE TO MOISTURE STRESS TOLERANCE IN MAIZE: BIOCHEMICAL VERIFICATION
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Date
2017-08-05
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Maize is an important highly productive cereal crop, but susceptible to drought
particularly during reproductive stage. In the present study, the pollen grains of
susceptible (BTM1 and BTM18) and resistant (BTM2 and BTM10) inbred lines were
subjected to osmotic stress (50 per cent PEG) under in vitro conditions. The size of the
pollen grains were reduced in all the inbred lines when exposed to osmotic stress.
However, the reduction was highest in BTM1 and least in BTM2. The pollen grains
regained their size when the stress medium was supplemented with the CaCl2. The
recovery was higher in BTM2 and lower in BTM1. The in vitro stressed pollen grains
were used for pollination of field grown plants but, they failed to produce seeds. In vivo
pollen selection by painting the stigma with PEG before pollination produced seeds. The
pollen biochemical response to stress was studied and increased activity of superoxide
dismutase and peroxidase was observed in stressed pollen grains and plants. The
increased activity was more in resistant pollen grains and plants compared to the
susceptible inbred line. No differences were found for isozyme banding pattern of
resistant and susceptible pollen grains and plants. Sixteen polymorphic SSR primers were
used to study genetic similarity of four inbred lines. In the dendrogram, the tolerant
inbred lines (BTM2 and BTM10) were clustered together.
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