IN VITRO SELECTION OF GINGER (ZINGIBER OFFICINALE ROSC.) AGAINST FUSARIUM OXYSPORUM f.sp. ZINGIBERI
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Date
2013
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Abstract
ABSTRACT
The present investigation aims at ‘In vitro selection of ginger (Zingiber officinale
Rosc.) against Fusarium oxysporum f.sp. zingiberi’. Callus was obtained from root
explants procured from in vitro cultures of ginger cv. Himgiri. MS medium
supplemented with 1.0 mg/l BA and 0.5 mg/l 2, 4-D proved to be the best medium for
callus induction. Shoot differentiation and regeneration from callus was achieved on MS
medium supplemented with 1.0 mg/l BA and 0.1 mg/l NAA. Well-developed multiple
shoots formed roots on the same medium after 2-3 passages of subculture, thus
eliminating the necessary step of in vitro rooting. Cell line selection against Fusarium
was done by using culture filtrate of Fusarium oxysporum f.sp. zingiberi as a selective
agent. Calli was selected at 15 per cent level of fungal culture filtrate and multiplied on
the previously standardized callus induction medium. Then the selected calli were
cultured on shoot differentiation medium. After shoot differentiation the rooted controls
as well as selected plantlets were tested through in vitro testing using mycelium
suspension of Fusarium oxysporum f.sp. zingiberi. One resistant plant could be obtained
after in vitro selection which showed slight symptoms of Fusarium infection which was
hardened by transferring to sterilized potting mixture.