STUDIES ON SOMATIC EMBRYOGENESIS IN PAPAYA CV. SURYA WITH REFERENCE TO ENDOPHYTIC BACTERIAL ASSOCIATION
| dc.contributor.advisor | PIOUS, THOMAS | |
| dc.contributor.author | PRABIR KUMAR, DAS | |
| dc.date.accessioned | 2016-12-15T10:38:32Z | |
| dc.date.available | 2016-12-15T10:38:32Z | |
| dc.date.issued | 40625 | |
| dc.description.abstract | Papaya (Carica papaya L.) is an important fruit crop world over. Micro propagation of shoot buds approach has not been very successful for commercial propagation purpose. Feasibility of somatic embryogenesis from immature zygotic embryos is also suggested in papaya. A protocol has now been optimized for somatic embryogenesis from immature zygotic embryos of papaya cv. Surya. Immature zygotic embryos were established on half-strength MS medium with supplements (MS vitamins, 6 per cent sucrose, 10 mg l-1 2, 4-D, 400 mg l-1 glutamine and 0.8 per cent agar (pH 5.7) giving high frequency and repetitive embryogenesis after 3-4 subculture passages. Continuous proliferation of somatic embryos was achieved on maturation and regeneration medium containing half-strength MS constituents, 3 per cent sucrose, 400 mg l-1 glutamine, 0.2 mg l-1 BAP and 0.1 mg l-1 NAA and 0.8 per cent agar. Medium containing a combination of 0.5 mg l-1 IAA and 0.5 mg l-1 IBA was ideal for root development. Endophytes are generally known to originate from the rhizosphere or phyllosphere and seeds are generally considered to be free from endophytes. Tissue and medium indexing on visibly clean embryogenic cultures initiated from immature seeds showed the association of cultivable bacteria in a small section (1.4 per cent) of cultures after three subculture passages. One bacterium was isolated from such cultures, which is identified as Brachybacterium rhamnosum based on 16S rRNA gene sequence homology analysis. PCR-screening of visibly clean and index negative cultures using universal bacterial 16S rRNA gene primers indicated the presence of non-cultivable bacteria in index-negative cultures. This was confirmed by microscopy of tissue homogenate revealing viable bacteria in considerable number. | en_US |
| dc.identifier.other | TH-10125 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/90254 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of Agricultural Sciences GKVK, Bangalore | en_US |
| dc.sub | Animal Biotechnology | en_US |
| dc.these.type | M.Sc | |
| dc.title | STUDIES ON SOMATIC EMBRYOGENESIS IN PAPAYA CV. SURYA WITH REFERENCE TO ENDOPHYTIC BACTERIAL ASSOCIATION | en_US |
| dc.type | Thesis | en_US |