Characterization of Lactic Acid Bacteria from the Gut of Penaeus vannamei as Potential Probiotic

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dc.contributor.authorLalitha, N.
dc.contributor.authorRonald, B.S.M.
dc.contributor.authorAnandachitra, M.
dc.contributor.authorHemalatha, S.
dc.contributor.authorSenthilkumar, T.M.A.
dc.contributor.authorMuralidhar, M.
dc.contributor.authorTANUVAS
dc.date.accessioned2023-03-17T10:33:37Z
dc.date.available2023-03-17T10:33:37Z
dc.date.issued2022-12
dc.descriptionTNV_IJAR_56(12)_Dec.2022_1499-1505en_US
dc.description.abstractBackground: The present objective of the study was to isolate and characterize the gut associated culturable lactic acid bacteria (beneficial bacteria) from the gut of Penaeus vannamei for their potential application as probiotic. Methods: Penaeus vannamei (host) gut associated bacterial isolates were obtained from ten commercial brackishwater shrimp ponds (n=10) located in Kanchipuram, Chengalpattu, Tiruvallur and Villupuram districts of Tamil Nadu, during 2021-22 to test their efficiency as indigenous gut probiotic. Twenty-five shrimps from each pond, with salinity ranging from 5 to 25 ppt, were collected for isolation of beneficial bacterial isolates. Result: Thirty lactic acid bacteria were isolated and identified from the gut of 250 Penaeus vannamei, using a 16S ribosomal DNA sequence. Six isolates viz., Pediococcus pentosaceus (ON495586), Lactiplantibacillus plantarum (ON491817), Lactococcus lactis (ON479264), Enterococcus faecium (ON478992), Enterococcus hirae (ON478991) and Enterococcus durans (ON564885) having better enzyme activity were taken and further subjected to in vitro analysis. It was found that these isolates had antibacterial activity against shrimp pathogens V. campbelli, V. harveyi and V. parahaemolyticus with zone of inhibition ranging between 12.33 to 21.00 mm; showed better growth at pH 7.0; tolerated the bile salts up to 1% concentration and endured salt concentrations up to 6.0%. In addition, above isolates demonstrated excellent auto-aggregative activity (74.45 to 91.14%) and hydrophobicity (77 to 99.93%). No antagonist activities were detected among the strains, suggesting its use as the multiple cocktail probiotic. Hence, the investigated isolates could serve as potential probiotics in shrimp aquaculture production systems.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810195377
dc.keywordsAquaculture, Lactic acid bacteria, Penaeus vannamei, Probioticen_US
dc.language.isoEnglishen_US
dc.pages1499-1505en_US
dc.publisherIndian Journal of Animal Researchen_US
dc.relation.ispartofseries;12
dc.subjectVeterinary Scienceen_US
dc.subjectVeterinary Pathologyen_US
dc.titleCharacterization of Lactic Acid Bacteria from the Gut of Penaeus vannamei as Potential Probioticen_US
dc.title.alternativeIndian Journal of Animal Researchen_US
dc.typeArticleen_US
dc.volume56en_US
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