Study on clinical application of exfoliative vaginal cytology in dog reproduction
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Date
2021-07-30
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Palampur
Abstract
The present study was conducted in she-dogs (N=30), belonging to different breeds
and ages, presented to the Department of Veterinary Gynaecology and Obstetrics, CSK
Himachal Pradesh Krishi Vishvavidyalaya (CSKHPKV), Palampur for determination of
mating time and diagnosis of various reproductive disorders. Serum progesterone was
estimatedfrom the blood sample collected in clot activator by cephalic venepuncture from
she-dogs during different stages of the estrous cycle. The objectives of the study were to
establish the usefulness of exfoliative vaginal cytology for the diagnosis of various
reproductive stages and disorders in she-dogs and compare different staining techniques
for vaginal cytology in canines.
All the staining techniques provided diagnostic results, however, the Giemsa
staining method proved to be the best followed by Leishman's method of staining. A
significantly higher proportion (p0.05) for non- cornified cells (r= -0.7408 to -0.5807) during early
estrus. Also, the percentage of keratinized cells and progesterone concentration were
significantly lower (p<0.01-0.05) in she–dogs subsequently conceived after mating as
compared to non-conceived.