Evaluation of methylotrophs for plant growth promotion of mungbean (Vigna radiata (L.) Wilczek) and urdbean (Vigna mungo (L.) hepper)
| dc.contributor.advisor | Leela Wati | |
| dc.contributor.author | Ahlawat, Umang | |
| dc.date.accessioned | 2017-12-06T06:32:50Z | |
| dc.date.available | 2017-12-06T06:32:50Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | Mungbean and Urdbean are two important pulses grown in India and they play an important role in human nutrition. Methylotrophic bacteria exert several beneficial effects on host plant such as stimulation of plant growth, nitrogen fixation and induction of resistance to plant pathogen. The use of methylotrophs offers an attractive way to replace chemical fertilizers, pesticides and supplements. In the present study, samples were collected from farm area of pulses section, Genetics and Plant Breeding, COA, CCS HAU, Hisar in summer and kharif season to isolate the methylotrophs. In total, 60 methylotrophs were isolated using ammonium mineral salt medium. After isolation, all the methylotrophic isolates were characterized for plant growth promoting traits: Indole acetic acid production, phosphate solubilization, siderophore production, ACC utilization, temperature tolerance (up to 450C) and salt (NaCl) tolerance (up to 5%). The majority of the methylotrophic isolates were found to be IAA producers, though the production varied in the range of 01.09-29.99μg/ml and the isolate MP12 produced maximum IAA (29.99 μg/ml) after 5 days incubation at 300C.P-solubilization index (P-S.I.) varied from 0.60 to 2.96 after four days of incubation at 30ºC and maximum P-SI was recorded in the isolate PP1(2.96). Siderophore production was detected by CAS (Chrome azurol S) assay and it was observed that out of 60 isolates only 9 methylotrophic isolates were siderophore producers. Overall 18% of methylotrophic isolates showed significant ACC utilization on minimal medium plates. Four promising methylotrophic isolates, namely MP12, MP15, UP4 and PP1 which showed maximum number of plant growth promoting traits were selected for plant growth evaluation of mungbean and urdbean under pot house conditions. Among various treatments, coinoculation of methylotrophic isolate MP12 along withRhizobium (MB703) and phosphate solubiliser (P36) was the best treatment with respect to nodulation, plant growth and yield attributes under pot house as well as field conditions. On the basis of morphological, biochemical characterization and 16S rDNA sequencing isolate MP12, PP1 and UP4 were identified to be Methylobacterium oryzae UALW_217, Ochrobactrum intermedium UALW_217 and Microbacterium ginsengisoli UALW_217. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810036998 | |
| dc.keywords | Mungbean, Urdbean, Methylotrophs, Nodules, Phyllosphere, Rhizosphere | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | CCSHAU | en_US |
| dc.sub | Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | Evaluation of methylotrophs for plant growth promotion of mungbean (Vigna radiata (L.) Wilczek) and urdbean (Vigna mungo (L.) hepper) | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | Evaluation of methylotrophs for plant growth promotion of mungbean (Vigna radiata (L.) Wilczek) and urdbean (Vigna mungo (L.) hepper) | en_US |
| dc.type | Thesis | en_US |