Bio-management of chickpea wilt incited by Fusarium oxysporum f.sp. ciceri (Padwick) Synd. & Hans.

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dc.contributor.advisorNarinder Singh
dc.contributor.authorArpanpreet Singh
dc.date.accessioned2021-09-03T05:00:07Z
dc.date.available2021-09-03T05:00:07Z
dc.date.issued2020
dc.description.abstractFusarium wilt of chickpea caused by Fusarium oxysporum f.sp. ciceri is a serious disease in chickpea growing areas of Punjab. Rhizosphere soil samples were collected from chickpea fields of Ludhiana, Gurdaspur, Mansa, Moga and Bathinda regions and analysed for antagonistic microflora. Five isolates of Trichoderma spp. and three isolates of Pseudomonas spp. were isolated and screened in vitro for antagonistic activity against Fusarium oxysporum f.sp. ciceri causing wilt of chickpea. Th5 isolate of Trichoderma viride and Pf2 isolate of Pseudomonas aeruginosa showing maximum inhibition of 73.11 and 67.63 per cent against Fusarium oxysporum f.sp. ciceri in dual culture assay were selected. Th5 isolate also showed highest chitinase activity 8.85 unit/ml and Pf2 showed highest siderophore activity with the production of 29.07 mm diameter of orange to yellow coloured halo. Efficacy of talc based bioformulations of Trichoderma viride and Pseudomonas aeruginosa isolates applied as seed, soil and seed + soil treatment was seen against Fusarium wilt of chickpea under field and pothouse conditions. Maximum disease inhibition was observed in chemical treatment (Carbendazim) with 88.95, 88.12 and 88.72 per cent in Ludhiana, Gurdaspur and Pothouse trial, respectively, followed by the Trichoderma viride (Th5) seed + soil treatment with 81.84, 85.69 and 83.63 per cent disease inhibition in Ludhiana, Gurdaspur and Pothouse trial, respectively. Maximum growth promotion was also recorded in chemical treatment (Carbendazim) i.e. shoot length, root length and ultimately increased the yield of chickpea under both field and pothouse conditions followed by the isolate Trichoderma viride (Th5) seed + soil treatment. The rhizospheric competence of the antagonists showed that enough population density of antagonists was present throughout the crop period to control the pathogen both under both field and pothouse conditions. Maximum shelf life of talc-based powder bioformulation of all the antagonists was recorded in low temperature conditions (0°C) when packaged in polythene bags.en_US
dc.identifier.citationArpanpreet Singh (2020). Bio-management of chickpea wilt incited by Fusarium oxysporum f.sp. ciceri (Padwick) Synd. & Hans. (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810175008
dc.keywordsFusarium wilt of Chickpea, Trichoderma viride, Pseudomonas aeruginosa, Bioformulationen_US
dc.language.isoEnglishen_US
dc.pages80en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemBio-management of chickpea wilt incited by Fusarium oxysporum f.sp. ciceri (Padwick) Synd. & Hans.en_US
dc.subPlant Pathologyen_US
dc.themeBio-management of chickpea wilt incited by Fusarium oxysporum f.sp. ciceri (Padwick) Synd. & Hans.en_US
dc.these.typeM.Scen_US
dc.titleBio-management of chickpea wilt incited by Fusarium oxysporum f.sp. ciceri (Padwick) Synd. & Hans.en_US
dc.typeThesisen_US
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