Bio-management of chickpea wilt incited by Fusarium oxysporum f.sp. ciceri (Padwick) Synd. & Hans.
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Date
2020
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Punjab Agricultural University, Ludhiana
Abstract
Fusarium wilt of chickpea caused by Fusarium oxysporum f.sp. ciceri is a serious disease in
chickpea growing areas of Punjab. Rhizosphere soil samples were collected from chickpea fields
of Ludhiana, Gurdaspur, Mansa, Moga and Bathinda regions and analysed for antagonistic
microflora. Five isolates of Trichoderma spp. and three isolates of Pseudomonas spp. were
isolated and screened in vitro for antagonistic activity against Fusarium oxysporum f.sp. ciceri
causing wilt of chickpea. Th5 isolate of Trichoderma viride and Pf2 isolate of Pseudomonas
aeruginosa showing maximum inhibition of 73.11 and 67.63 per cent against Fusarium
oxysporum f.sp. ciceri in dual culture assay were selected. Th5 isolate also showed highest
chitinase activity 8.85 unit/ml and Pf2 showed highest siderophore activity with the production of
29.07 mm diameter of orange to yellow coloured halo. Efficacy of talc based bioformulations of
Trichoderma viride and Pseudomonas aeruginosa isolates applied as seed, soil and seed + soil
treatment was seen against Fusarium wilt of chickpea under field and pothouse conditions.
Maximum disease inhibition was observed in chemical treatment (Carbendazim) with 88.95, 88.12
and 88.72 per cent in Ludhiana, Gurdaspur and Pothouse trial, respectively, followed by the
Trichoderma viride (Th5) seed + soil treatment with 81.84, 85.69 and 83.63 per cent disease
inhibition in Ludhiana, Gurdaspur and Pothouse trial, respectively. Maximum growth promotion
was also recorded in chemical treatment (Carbendazim) i.e. shoot length, root length and
ultimately increased the yield of chickpea under both field and pothouse conditions followed by
the isolate Trichoderma viride (Th5) seed + soil treatment. The rhizospheric competence of the
antagonists showed that enough population density of antagonists was present throughout the crop
period to control the pathogen both under both field and pothouse conditions. Maximum shelf life
of talc-based powder bioformulation of all the antagonists was recorded in low temperature
conditions (0°C) when packaged in polythene bags.
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Citation
Arpanpreet Singh (2020). Bio-management of chickpea wilt incited by Fusarium oxysporum f.sp. ciceri (Padwick) Synd. & Hans. (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.