ISOLATION, CHARACTERIZATION AND ANTIBIOGRAM PATTERN OF SALMONELLA AND ESCHERICHIA COLI IN ENTERIC INFECTIONS OF PIGS IN ANDHRA PRADESH
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Date
2008-12
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
Multiple serovars of Salmonella and serotypes of E. coli are responsible to cause severe infections in pigs leading to morbidity and mortality and economic loss to swine industry. These bacteria also affect human health and have public health impact. Isolation and identification of indigenous Salmonella serovars / E. coli serotypes is of prime importance in order to carryout research on development of vaccines and for the study of molecular epidemiology. Hence, an attempt was made in this direction in the present study. Among 101 faecal samples screened for salmonellosis the notable serovars Salmonella Derby, S. Typhimurium and S. Typhisuis surfaced as major incriminating agents in pigs while the E. coli serotypes identified were O1, O2, O5, O8, O10, O12, O22, O24, O60, O88, O89, O92, O123, O138, O154 and O159. The bacterial aetiology was confirmed with standard procedures and protocols of Bergey’s manual of determinative bacteriology, ninth edition. The haemagglutination pattern of Salmonella and E. coli exhibited agglutination of erythrocytes viz., cattle, sheep, goat, chicken, rabbit, guinea pig and mice indicating the importance of fimbriae in colonization of organisms in intestine and production of toxins. Anti-sensi disk patterns revealed that the drugs of choice were chloramphenicol, co-trimoxazole and gentamicin for Salmonella infection while gentamicin, amoxycillin and chloramphenicol were the suitable drugs for chemotherapy of E. coli infection.
The in vivo pathogenicity studies in mice revealed that S. Typhimurium was most pathogenic followed by S. Derby and S. Typhisuis whereas the
pathogenicity studies carried out in chicks with E. coli serotypes viz., O1, O2, O8, O10, O22, O88, O92, O123, O138, O138 and O159 produced characteristic lesions and mortality in affected chicks. An innovative study on effect of Salmonella serovars viz., S. Typhimurium and S. Derby and E. coli serotypes O1, O24, O60 O89, O92, O123, O138 and O159 on germination of seeds were found to be toxic causing the inhibition of germination. Cefotaxime extract of Salmonella serovars on SDS-PAGE yielded 11 polypeptide bands ranging in molecular weight from 14 to 95 kDa while fimbrial extract of E. coli revealed a single band of 18 kDa.
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