BIOACTIVE ATTRIBUTES OF HERBAL SUPPLEMENTED FERMENTED MILK FROM INDIGENOUS CATTLE
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Date
2020-10-01
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CSKHPKV Palampur
Abstract
Milk supplementation with aqueous extracts of herbal species increases the consistency of the dairy products. In the current study, the probiotic Lactobacillus rhamnosus GG (LGG)-fermented indigenous hill cattle milk, pepsin-digested and overnight digested milk supplemented with aqueous herbal extracts (1%) of fruits of harad (Terminalia chebula), baheda (Terminalia bellirica), amla (Emblica officinalis) and bark of arjuna (Terminalia arjuna) were evaluated for antioxidative, antimicrobial and angiotensin converting enzyme (ACE) inhibitory activities. Fermented milk was prepared by adding 10 ml of different aqueous herbal extracts to 85 ml of fresh boiled indigenous cattle milk and 5 g of LGG as starter culture. Total phenolic content was significantly (P<0.05) higher in fermented milk containing aqueous baheda extract in undigested (26.43±1.16 mgTAE/100ml), pepsin-digested (36.53±0.30 mgTAE/100ml) and overnight digested samples (33.98±0.26 mgTAE/100ml). Ferric reducing antioxidant power (FRAP) was significantly (P<0.05) higher in fermented milk containing aqueous extract of harad in undigested (247.46±1.38 mg FeSO4 equivalent/100ml), pepsin-digested (266.21±0.38 mg FeSO4 equivalent/100ml) and overnight digested samples (235.64±0.62 mg FeSO4 equivalent/100ml). DPPH activity was found to be highest in undigested and pepsin-digested samples of fermented milk containing aqueous baheda extract (26.40±0.94% and 39.66±0.44%). However, DPPH activity was found to be highest in overnight digested fermented milk containing aqueous amla extract (27.31±0.48%). Maximum ACE inhibitory activity was noted in fermented milk containing aqueous arjuna extract (19.70±0.58%). The overnight digested samples demonstrated higher ACE inhibitory activity in fermented milk containing aqueous baheda extract (49.79±0.23%). Antimicrobial activity against Bacillus cereus and Rhodococcus equi was seen in fermented milk containing various aqueous herbal extracts. Prominent peptide bands were visible in milk samples prior to centrifugation, and no peptide bands were visible in centrifuged samples. It is inferred that herbal extracts fermented milk with probiotic LGG had better antioxidant, antimicrobial and angiotensin converting enzyme (ACE) inhibitory activities.