MOLECULAR EVALUATION OF ADVANCED BACKCROSS PROGENIES FOR SALINITY TOLERANCE IN RICE (Oryza sativa L.)
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Date
2015
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Acharya N.G. Ranga Agricultural University, Guntur
Abstract
The present investigation was undertaken with an aim to screen and identify the advanced breeding lines for salinity tolerance through genotyping and phenotyping, identification of salinity tolerant lines.
Eighty families of BC3F2 population of the cross MTU 1010 x FL 478 with 108 individual plants were evaluated. Out of the 8,640 plants evaluated based on morphological similarity with recurrent parent a total of 3,317 plants were selected for genotyping studies. In foreground selection using RM 10694, out of 3,317 plants 728 plants were having Saltol, a major QTL for seedling stage salinity tolerance. In recombinant selection AP 3206 and RM 10793 were used and out of the 728 plants screened, 244 plants from 69 families were double recombinants which were further studied for salinity tolerance in hydroponics.
The total 244 double recombinants selected after foreground and recombinant selection were phenotyped using hydroponic study for salinity tolerance. After hydroponics a total of 116 plants survived after transferring to main field. In confirmation studies under foreground selection, 79 plants from 13 families having Saltol were selected. In recombinant selection, out of the 79 plants screened, 69 plants were double recombinants.
Correlation studies were taken up in the survived lines for the yield and its contributing traits with salinity tolerance. The association between grain yield/plant and initial and final salinity scores was negative and significant. The correlation of initial salinity score was negative with ear bearing tillers/plant, number of filled grains/panicle, spikelet fertility and test weight. Final salinity score exhibited
negative correlation with all the yield attributing traits. Hence, in the present study after genotyping and phenotyping, a total of 69 advanced back cross lines with salinity tolerance were identified. These lines can be further multiplied and tested in yield trials and an alternate variety to MTU 1010 with salinity tolerance can be developed.
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D5183
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