Assessment of genetic diversity in garden pea (Pisum sativum L.) using agro-morphological and molecular markers

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dc.contributor.advisorSharma, Akhilesh
dc.contributor.authorSharma, Shimalika
dc.date.accessioned2022-02-17T10:52:54Z
dc.date.available2022-02-17T10:52:54Z
dc.date.issued2021-10-26
dc.description.abstractThe present investigation was undertaken to evaluate the morphological and molecular diversity among the garden pea genotypes during winters 2019-20 and 2020-21. The experimental material comprising of 56 genotypes was evaluated in randomized completeblock design with three replications and data were recorded on pod yield per plant along with component traits. Sufficient genetic variability was observed for all morphological, yield and yield contributing, and quality traits during both the years of 2019- 20 and 2020-21. Genotypes SP-6, SP-22, SP-3, 2018/PMVAR/1 and 2019/PMVAR/1 were observed to be top ranking five promising genotype on the basis of yield and related traits. High heritability coupled with high genetic advance was observed for pod yield per plant which revealed the importance of additive gene action and phenotypic selection would be effective for improvement in the early generations. Correlation and path coefficient analysis revealed that pods per plant, average pod weight, shelling (%) and seeds per pod could be considered as the best selection parameters for evolving high yielding genotypes. The multivariate analysis revealed considerable genetic diversity present in the 56 genotypes studied and were arranged into 8, 5 and 6 clusters in 2019-20, 2020-21 and pooled over years, respectively. Number of pods per plant contributed maximum towards total genetic divergence during both the years and pooled over years, respectively. By virtue of desirable characters as well as maximum inter-cluster distance genotypes SP-6, SP-3, SP-22, SP-12, SN-8-2, DPPMFWR-30, Pusa Shree, Matar Ageta, Palam Triloki, Palam Priya, SN-2, SP-18, 2019/PMVAR/6 and Palam Sumool were identified. Molecular diversity analysis using UPGMA method indicated the presence of considerable diversity in the population as genotypes were grouped into two main clusters. Twelve genotypes viz., 2019/PMVAR/1, 2019/PMVAR/7, 2017/PMVAR/5, 2018/PMVAR/1, 2017/PMVAR/6, 2018/PMVAR/5, Pb89, SN-5, SP-18, SN-6, SP-10 and SN-10 were found common while comparing morphological and SSR data and, thereby exhibiting the consonance between morphological and SSR data. These genotypes offera scope for utilization in hybridization as parents for the isolation of transgressive segregants in garden pea.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810182460
dc.keywordsgenotypes,generations,plant,diversity.en_US
dc.language.isoEnglishen_US
dc.pages183en_US
dc.publisherPalampuren_US
dc.subVegetable Scienceen_US
dc.themeTo assess the genetic diversity among garden pea genotypes based on agro-morphological and molecular markers and to estimate the genetic parameters of variability, association among traits andtheir direct and indirect contributions towards pod yield.en_US
dc.these.typeM.Scen_US
dc.titleAssessment of genetic diversity in garden pea (Pisum sativum L.) using agro-morphological and molecular markersen_US
dc.typeThesisen_US
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