“Clonal propagation and bioprospection studies of marchantia spp.”
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Date
2022-09
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G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145
Abstract
Bryophytes are first successful land colonising plant community. They form second largest taxonomic category of plants after angiosperms. Although, these are rich in biochemical compounds, yet their chemistry has been negligibly explored much due to habitat specificity and low sample availability. The present study consists of two major experiments. First experiment investigated theprotocol for mass multiplication of one of the most important genera of bryophytes i.e., Marchantia. Lab cultivation of 2 species of Marchantia, M. polymorpha and M.papillata was successfully done through in vitro MS medium culture and ex vitro soil culture respectively. Gemmae (surface sterilized with 1% sodium hypochlorite) were used as an explant for in-vitro culture of M. polymorphaonhalf strength MS mediumw/o sucrose.Among all tested lights, nutrients and temperature conditions, optimum thallus proliferation was seen in white light at 20°C. For ex vitro soil culture of M. papillata, treatment with rice-washed water in soil,sand,cocopeat and vermicompost mixture showed best results for thallus proliferation. Lab established clonal population of M. papillata was bio prospected for various biological activities and results were compared with those of the wild population in the second experiment. Methanolic and aqueous extract was subjected to both enzymatic and non-enzymatic antioxidant assays. Anti-inflammatory activity and anti-diabetic activity was measured by egg-albumin protein denaturation assay and α- amylase inhibitory assay respectively. Methanolic extracts of lab propagated plants exhibited higher antioxidant and anti-diabetic activity whereas methanolic extract of wild population exhibited higher anti-inflammatory activity. The aqueous extract of lab propagated plants showed highest herbicidal activity. The GC-MS analysis of methanolic extracts of wild and labgrown M. papillata majorly showed the presence of methyl esters, phytol, linoleic acid and sesquiterpenoids etc., contributing to the pharmacological effects such as antioxidant, anti-inflammatory, anti-tumor, antidiabetic etc.Comprehensively, this study allows to obtain the monocultured sample availability all around the year resulting in the conservation of species as well as usage of in-vitroand ex vitro grown axenic populationsas a substitute of wild populations of for research and clinical purposes.