Evaluation of haemato-biochemical profile and anti-osteoporotic potential of Moringa oleifera in rats
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Date
2019-03
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present study was carried out to evaluate haemato-biochemical profile and antiosteoporotic potential of Moringa oleifera in combination with prednisolone induced osteopororsis in rats. Osteopororsis was induced in rats by administration of prednisolone @ 1mg/ kg, bwt, I.P, daily for 28 days under aseptic conditions. Rats were divided in 6 groups of six animals each. Group I served as control, group II and group III were administered aqueous extract of Moringa oleifera (MOAE) @ 200mg/kg b. wt and 500mg/kg b.wt repectively. In group IV and V aqueous extract of Moringa oleifera (MOAE) @ 200mg/kg b. wt and 500mg/kg b.wt with prednisolone @ 1mg/kg b wt and in group VI prednisolone was administered @ 1mg/kg b wt. daily for 28 days. The antiosteoporotic potential was measured by determining bone histomorphometry by X-ray radiography of femur of rats. Osteoporotic biomarkers were recorded by determining alkaline phosphatase and serum calcium. Osteoporotic biomarkers such as ALP and calcium were reduced after treatment with prednisolone. Effect on hepatorenal function was assessed by determining effect on AST, creatinine and BUN level in serum. Treatment with MOAE-1 and MOAE-2 with prednisolone treated rats restored the blood urea nitrogen level and creatinine level. There was signinficant (P<0.05) alteration in body weight, femur weight and radiographic examination which indicated the induction of osteoporosis after 28 days in rats.. In groups treated with MOAE-1 and MOAE-2 with prednisolone showed improvement in level of calcium as compared to control after 14th and 28th day of treatment. In prednisolone treated group dislocation of femur head from pelvic girdle and fracture in mid shaft of femur were observed , which conforms osteoporosis in prednisolone treated group. osteoporosis very often manifests as a bone fracture. Femoral fractures are the most feared complication of osteoporosis. Prednisolone altered hematological parameters like Hb, PCV, TLC, TEC and DLC , effect was ameliorated by treatment with aqueous extract of Moringa oleifera @ 200 and 500 mg/kg b.wt for 28 days. Histopathological changes in liver, kidney and spleen were observed in prednisolone treated groups , and groups including prednisolone with MOAE-1 and MOAE-2. Thus, it can be concluded from present study that the aqueous extract of Moringa oleifera has antiosteoporortic potential against prednisolone induced osteoporosis
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