Development of swift diagnostic PCR Assay for molecular detection of Clostridium novyi type B in cattle

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dc.contributor.authorPuvarajan, B
dc.contributor.authorLurthu Reetha, T
dc.contributor.authorSelvaraj, J
dc.contributor.authorManickam, R
dc.contributor.authorTANUVAS
dc.date.accessioned2020-03-09T10:05:20Z
dc.date.available2020-03-09T10:05:20Z
dc.date.issued2020-02
dc.descriptionTNV_20thMVC_PP_Feb-2020_PA36en_US
dc.description.abstractClostridium novyi type B causes infectious necrotic hepatitis (INH) in sheep and less frequently in other species of livestock. A 7 year old Gir cow after postmortem examination revealed icterus with large amount of serosanguineous fluid in the abdominal cavity and the liver samples were received for bacteriological examination and was subjected to anerobic cultivation followed by biochemical characterization in association with Grams staining which was identified as Clostridium novyi. Further the liver tissue was subjected to PCR and were found positive for C. novyi type B alpha toxin genes but negative for Clostridium haemolyticum andother clostridia. Histologically, there was coagulative necrosis surrounded by a rim of inflammatory cells and large numbers of gram-positive rods and concluded as a definitive diagnosis of infectious necrotic hepatitis (INH).In ruminants, also known as black disease, INH has rarely been reported in cattle . An amplicon of 427 bp corresponding to 16SrRNA gene sequence was obtained and this PCR Assay was standardized as this was confirmed simultaneously with the type culture DNA extract (ATCC 25758). Moreover owing to the Clostridial infections in cattle causing acute death, this is the first report of identifying the Clostridium novyi through molecular means of earlier detection paving way as an efficient tool for precise diagnosis of Clostridium novyi type B infections in cattle.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810145077
dc.keywords20th Indian Veterinary Congress, Annual Conference of IAAVR, TANUVASen_US
dc.language.isoenen_US
dc.pages1en_US
dc.subjectVeterinary Scienceen_US
dc.titleDevelopment of swift diagnostic PCR Assay for molecular detection of Clostridium novyi type B in cattleen_US
dc.title.alternativeNational Symposium on Veterinary Research Priorities in Translational Animal Health, Production and Food Safetyen_US
dc.typePresentationen_US
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