DETECTION OF ENTERIC DNA VIRUSES FROM DIARRHEIC DOGS OF HIMACHAL PRADESH.
dc.contributor.advisor | Dhar, Prasenjit | |
dc.contributor.author | Thakur, Deepika | |
dc.date.accessioned | 2019-11-02T06:43:03Z | |
dc.date.available | 2019-11-02T06:43:03Z | |
dc.date.issued | 2019-07 | |
dc.description.abstract | Diarrhea is among the most common clinical conditions seen in dogs leading to high morbidity and mortality. It can have various causes, including infectious agents, like viruses, bacteria and parasites. The aim of the study was to isolate enteric DNA viruses from diarrheic dogs of Himachal Pradesh and characterize them at molecular level. A total of 238 fecal/other samples were collected from diarrheic / apparently healthy dogs along with brief clinical history of the animal. The faecal samples were subjected to DNA extraction and PCR for the detection of enteric DNA viruses viz. CPV, CAV-1 and Dog CV with primers targeting the VP2, pⅦ and replicase gene of CPV, CAV-1 and Dog CV respectively of each virus were used in the study. 109 (45.79 %) samples were found positive for CPV-2b and 4 (1.68%) samples were found positive for CAV-1. No samples were found positive for Dog CV. HA along with HI and Dot- ELISA were standardized for virus detection from clinical samples and infected cell culture fluids using hyper immune sera raised in rabbits against CPV and CAV-1. Dot-ELISA was found to be almost similar to in sensitivity HA-HI for detection of viruses. Growth studies of CPV and CAV- 1 were done on MDCK, A-72 and Vero cells. CPV could be adapted to MDCK and A-72 cells but not to Vero cells while adaptation of CAV-1 was not possible on any cell culture. Physicochemical characterization of CPV was done by checking the effect of temperature, pH, chloroform and formalin on the virus. The VP2 (CPV-2) and pⅦ (CAV-1) gene fragment was sequenced directly which revealed close genetic similarity to Indian and Italian isolates of CPV and CAV, respectively. Microbiological studies on fecal samples revealed 37 bacterial isolates that were resistanct to penicillin and streptomycin in antibiogram and were found concurrently associated with clinical cases of viral diarrhea. In conclusion, diarrhea in dogs in H.P are mostly caused by CPV type 2b strain and occasionally by CAV-1 and is frequently associated with other bacterial species. | en_US |
dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810133815 | |
dc.keywords | Dogs | en_US |
dc.language.iso | en | en_US |
dc.pages | 103p | en_US |
dc.publisher | CSKHPKV, Palampur | en_US |
dc.sub | Veterinary Microbiology | en_US |
dc.subject | null | en_US |
dc.theme | To isolate enteric DNA viruses from cases of diarrhea in dogs. | en_US |
dc.these.type | M.V.Sc. | en_US |
dc.title | DETECTION OF ENTERIC DNA VIRUSES FROM DIARRHEIC DOGS OF HIMACHAL PRADESH. | en_US |
dc.type | Thesis | en_US |
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