Analysis Of ß-Casein Gene For A1 And A2 Genotype Using Allele Specific PCR in Kangeyam And Holstein Friesian Crossbred Cattle in Tamil Nadu
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Date
2014-07
Journal Title
Journal ISSN
Volume Title
Publisher
TANUVAS, Chennai
Abstract
Milk is the one of the most important protein diet to the human
population. However, in last few decades, presence of A1 b casein in milk was
associated with important issues associated with range of illnesses in human
being. In this study a total of 85 cattle blood samples (Kangeyam and HF
crossbred) were analysed for A1 b casein gene based on AS-PCR. A1/A2
genotype frequency data indicated that 37% were A2 homozygous (A2A2),
17% were A1 homozygous (A1A1) and 46% heterozygous (A1A2) in HF cross
bred cattle. The pure Kangeyam (Bos indicus) cattle breed had only A2 gene
and showed only A2A2 genotype, which produce safer A2 milk for the human
consumption. The Holstein Friesian cross breed animal also showed mostly of
A2 gene (0.595).
Description
Keywords
Veterinary Science, Animal Biotechnology