STUDIES ON CHARACTERIZATION OF GENOTYPES, SEED PRODUCTION AND SEED STORABILITY OF CLUSTER BEAN (Cyamopsis tetragonoloba (L.) Taub.)
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Date
2015-07-20
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Studies on characterization of genotypes, seed fortification and seed storage were conducted during kharif 2013 and 2014. The genotype PNB was classified as glabrous and remaining all genotypes as pubescent. The genotype GG-2 had white coloured flowers while other genotypes had light purple coloured flowers. Based on the zymogram of total soluble proteins, Region D (29.0 to 43.0 KD), E (20.0 to 29.0 KD), F (14.3 to 29.0 KD) and G (< 14.3 KD) were found more useful to distinguish most guar genotypes. The isozyme electrophoresis of esterase produced marked differences in the banding pattern that could be used for the identification of genotypes. Ten RAPD primers revealed 96.7 per cent polymorphism and primer OPQ-7 was found to be highly polymorphic. Highly distinct genotypes among the selected germplasm were IC-102853 with IC-116594 followed by IC-102853 with IC-116626; HG-365 with IC-116594. Among the genotypes, HG-365 is high yielding and suitable for Pavagada location. Seeds fortified with MgS04 @ 1 per cent recorded higher plant growth, seed yield and quality parameters. The improvement in seed yield due to MgS04 fortification was up to 22.21 per cent than control. Seeds treated with spinosad (0.04 ml/kg) and stored in super grain bag recorded higher germination (80.00 %) and other seed quality parameters at the end of 18 months of storage.
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