Induction of resistance for the management of Alternaria leaf spot of cauliflower

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dc.contributor.advisorSharma, Monica
dc.contributor.authorSharma, Shalini
dc.date.accessioned2022-02-15T07:24:29Z
dc.date.available2022-02-15T07:24:29Z
dc.date.issued2020-10-18
dc.description.abstractThe pathogen causing Alternaria leaf spot of cauliflower was isolated and identified as Alternaria brassicicola on the basis of morphological characters and sequencing of DNA region coding for internal transcribed spacer (ITS) region. Amplification of genomic DNA of fungus with primer pair ITS1 and ITS 4 gave a single band of approximately 500 bp on gel electrophoresis. In pathogenicity test, 72 hours of incubation period of A. brassicicola was observed by spray inoculation of the pathogen. Among eight different abiotic resistance inducers, foliar sprays of salicylic acid (20 mM) and potassium chloride (100 mM) was found most effective for the management of Alternaria leaf spot of cauliflower in pot and field experiment. Under field conditions, three foliar sprays of salicylic acid (20 mM) and potassium chloride (100mM) resulted in reduction in disease incidence up to 76.81 and 71.01 per cent and disease index up to 78.69 and 73.36 per cent, respectively with maximum curd yield of 2.68 and 2.37 kg/plot. The phenol content and activity of polyphenol oxidase and peroxidase was maximum after three days of pathogen inoculation which then gradually diluted with time. Among aqueous extracts, maximum mycelial inhibition of 65.47 per cent was observed by using extract of Phyllanthus emblica at 50 per cent concentration. Cow urine alone resulted in complete inhibition in mycelial growth of pathogen at different tested concentrations i.e 10, 15 and 20 per cent. While, in cow urine based extracts, maximum mycelial inhibition of 53.33 and 51.67 per cent was observed by using extract of Ocimum tenuiflorum and Phyllanthus emblica at 20 per cent concentration. In dual culture assay, under in vitro conditions, Trichoderma harzianum I-6 strain resulted in maximum mycelial inhibition of 80.64 per cent followed by T. viride (78.49 %). Root dip treatment of cauliflower seedlings with T. harzianum I-6 strain was also found most effective with reduction of 32.70 and 40.00 per cent disease incidence and per cent disease index, respectivelyen_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810182302
dc.keywordsInduced resistance, Alternaria leaf spot of caulifloweren_US
dc.language.isoEnglishen_US
dc.pages92+ven_US
dc.publisherCOLLEGE OF HORTICULTURE AND FORESTRY, DR Y S P UHF, NERI, HAMIRPURen_US
dc.subPlant Pathologyen_US
dc.themeInduction of resistance for the management of Alternaria leaf spot of caulifloweren_US
dc.these.typeM.Scen_US
dc.titleInduction of resistance for the management of Alternaria leaf spot of caulifloweren_US
dc.typeThesisen_US
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Shalini Sharma; Monica Sharma
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