STUDIES ON BIOCHEMICAL CHANGES AND SEED QUALITY AS INFLUENCED BY OSMOCONDITIONING IN ONION (Allium cepa L.)
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Date
2007-08-21
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University of Agricultural Sciences GKVK, Bangalore
Abstract
The seeds of two cultivars of onion, viz., Arka Kalyan and Rampur
Rose were subjected to acceralated ageing at 40°C and 100 per cent RH
under laboratory conditions for 0 & 8 days to create two vigour levels
viz., high (non-aged) and low vigour seeds. Then they were osmoprimed
with PEG-6000 at -1.5 MPa & -1.0 MPa for 5 days and 7 days at 10°C in
order to assess the ability of priming treatments in repair of deterioration
sustained during ageing.
In the present investigation, it was observed that the PEG priming
had increased the seed quality parameters such as germination
percentage, mean seedling dry weight, SVI and the biochemical
parameters, viz., total soluble sugar, protein and lipid content; total
dehydrogenase and β-amylase activities in the seeds; but decreased the
EC, total soluble sugar and protein content in the seed leachate; MDA
content in the onion seeds of both the cultivars namely, Rampur Rose
and Arka Kalyan studied. Further, the SDS-PAGE banding pattern of
soluble seed protein profiles showed more variation with respect to band
number and intensity in Rampur Rose as compared to that of Arka
Kalyan upon PEG priming.
In overall, it can be concluded that the high vigour seeds of
Rampur Rose showed better improvement with regard to seed and
seedling vigour upon PEG treatment at -1.0 MPa for 7 days as compared
to other treatments.
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