Genetics of resistance and molecular analysis of botrytis grey mould in chickpea (Cicer arietinum L.)
| dc.contributor.advisor | Panwar, R.K. | |
| dc.contributor.author | Bhardwaj, Rajneesh | |
| dc.date.accessioned | 2019-02-05T06:24:28Z | |
| dc.date.available | 2019-02-05T06:24:28Z | |
| dc.date.issued | 2018-08 | |
| dc.description.abstract | Chickpea (Cicer arietinum L.) is the second largest consumed pulse crop of the world after common bean and it is grown in over 50 countries and traded across 140 countries. The beneficial effects of chickpea on soil health and human health are well recognized. There has been a slow progress in improving the average global productivity of chickpea, which continued to remain below 1.0-ton ha-1. In India, chickpea was planted in 8.25 million hectares during 2016-17 with a production of 7.33 million tons and a productivity of 859 kg/ha. However, the breakthrough in its productivity is still awaited because of the number of biotic and abiotic stresses constrain productivity. Botrytis gray mould (BGM) caused by Botrytis cinerea is one of the major constraints for low yield of chickpea. This disease leads to subsequent crop loss in certain parts of India (in Tarai area of Uttarakhand, Tal area of Bihar and in Punjab). The present investigation was carried out in Rabi seasons during 2014-15, 2015-16 and 2016-17 at N. E. Borlaug Crop Research Centre and Pulse Breeding Laboratory of G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India with the objectives to study the genetics of resistance to botrytis grey mould (BGM) disease and molecular analysis of BGM disease in chickpea. The experimental material comprised of resistant parents viz., GL10006, DKG876 and susceptible parents viz., DCP92-3, GNG1581 and H 208. Screening for resistance to botrytis grey mould disease was done by using field screening technique which involves spraying artificial inoculums of a pathogen. Disease scoring was done using the 1-9 scale. Resistance was found dominant over susceptibility in the F1 generation of six resistant × susceptible crosses viz. GL10006×GNG1581, GL10006×DCP92-3, GL10006×H208, DKG876×GNG1581, DKG876×DCP92-3 and DKG876×H208. In the F2 generation, 3(resistant): 1(susceptible) ratio was found for all resistant × susceptible crosses indicating that resistance was governed by a single dominant gene. Backcross of F1 with resistant parents gave all resistant plants and backcross with susceptible parent gave segregants into 1(resistant): 1(susceptible) ratio. The crosses between resistant (GL10006) × resistant (DKG876) parents (test of allelism) suggested that the dominant resistance gene in both the parents were different as F2 generation of this cross showed segregation in15:1 ratio. In another experiment, STMS markers were tested for their association with botrytis grey mould resistance genes in parents, F1 and the F2 population of cross GL10006 (resistant parent) × H208 (susceptible parent) of chickpea. In preliminary studies, total 28 STMS was used out of which 13 primers viz., TA203, TA47, TA43, TS9, TAA137, ICCM068, ICCM0160, TA34, TS72, TA118, TS57, TA25 and TA144 were identified with polymorphic bands in the resistant and susceptible parent. These polymorphic primers were also screened in the F1 and F2 population. In F1 plants, amplified band size was similar to the resistant parent. Plants in F2 generation have amplified bands of either resistant or susceptible parents because in the F2 generation, plants were segregated into resistant and susceptible plants. To identify the putative marker-trait association, the genotypic and phenotypic data of cross between resistant parent (GL10006) and susceptible parent (H208) were subjected to single marker analysis. Results of single marker analysis indicated that five markers ICM0068, TS72, TA118, TA25 and TA144 were associated with BGM resistance. BGM resistance locus (QTL) associated with TA118 primer explained the most 28.25% of total phenotypic variation for BGM resistance. Whereas TA25, TA144, TS72, ICM0068 explained 0.05%, 5.5%, 7.85%, 0.39% of total phenotypic variation for BGM resistance, respectively. Polymorphic markers identified in the present study will be useful for identification of markers linked with BGM resistance which can further assist in marker-assisted selection for resistance breeding. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810093635 | |
| dc.keywords | genetics, disease resistance, molecular genetics, Botrytis, moulds, chickpeas | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 103 | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.research.problem | Chickpeas | en_US |
| dc.sub | Genetics and Plant Breeding | en_US |
| dc.subject | null | en_US |
| dc.theme | Moulds | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | Genetics of resistance and molecular analysis of botrytis grey mould in chickpea (Cicer arietinum L.) | en_US |
| dc.type | Thesis | en_US |