PHYSICAL AND BIOCHEMICAL CHARACTERS ASSOCIATED WITH RESISTANCE TO BRUCHID Callosobruchus theobromae L. ON FIELDBEAN (Dolichos lablab L.)
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Date
40865
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University of Agricultural Sciences GKVK, Bangalore
Abstract
The investigations on screening of accessions of fieldbean, physical characters of pods, seeds and their biochemical constituents for resistance to bruchid, Callosobruchus theobromae L., were conducted at GKVK, University of Agricultural Sciences, Bangalore during 2009-2010. Forty fieldbean accessions were grown in the field to find out the resistant entry to pulse beetle C. theobromae infestation. The pod infestation ranged from 1.25 to 36.84 per cent. The entry GL-164 had recorded least pod damage (1.25%) among the 40 accessions followed by GL-78 (2.57%) and these were identified as tolerant accessions. Whereas, the accession HA-4 had recorded highest per cent pod infestation (36.84 %) followed by HA-3 (35.06%) and these were identified as susceptible. Different pod and seed characters like pod shape, seed colour and pod wall thickness were having positive influence on the pod infestation. Tolerant accession GL-164 recorded the lowest protein content (121.6 and 134.4 mg/g), total soluble sugar (18 and 28 mg/per g) highest phenol (7.12 and 8.56 mg/g), tannin (7.1 and 5.2 mg/g) in pod wall and seeds, respectively. Accession HA-4 recorded highest protein content (185.36 and 195.2 mg/g), total soluble sugar (65 and 85 mg/g) where as lowest phenol (3.87 and 4.22 mg/r g), tannin (2.3 and 1.00 mg/g) in pod wall and seeds, respectively. Pods of GL-164 recorded the highest enzymes activity of peroxidase (3900 units/g) and phenylalanine ammonia lyase (PAL) (168 units/g) whereas pods of HA-4 recorded lowest peroxidase (900 units/g) and (PAL) (87 units/g). The tolerant accessions GL-164, GL-187, GL-63, GL-195 and GL-127 have shown maximum of 2, 3, 5, 3 and 9 bands, respectively while the susceptible ones namely GL-36, GL-46, GL-102 HA-3 and HA-4 have shown a maximum of 11, 10, 9, 11, and 8 bands each. The bands 6 and 7 were commonly present in all the ten accessions.