CLONING AND CHARACTERIZATION OF KALA4 GENES IN MANIPURI BLACK RICE (Oryza sativa l.)
Loading...
Date
2016
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
World food security is becoming an ever more serious problem due to continuing population growth
and increasing competition for arable land between food and energy crops. In Asia, it serves as the
major source of energy, protein, thiamine, riboflavin, niacin, iron (Fe), zinc (Zn) and calcium (Ca) in
the diet. Nutritional quality of rice has received more attentionin the developing countries, where
monotonous consumption ofrice may lead to deficiencies of essential minerals, vitamins, andother
nutritional compositions. Black rice is a type of the rice species Oryza sativa L. which is glutinous,
packed with high level of nutrients and mainly cultivated in Asia. Coloured rice are reported to be
potent sources of antioxidants and their consumption as one of the sources is encouraged. Therefore,
characterization of genes responsible for colour in rice grain is important. In the present study, a
known gene Kala4 gene responsible for black colour in grain was sued to understand the molecular
mechanism for anthocyanin in rice grain. This gene was found to be 315 amino acids with
TRANSPARENT TESTA 8. The gene is about 24227 nucleotides length with 984 nucleotides of
cDNA and having 7 exons and 5 introns. A total of four SSR markers were developed from this gene
and association analysis in 23 diverse coloured rice found to be non-significant association.
Detection of functional domain of helix loop helix domain (HLH) structure indicated the importance
role in the gene regulation. The protein was predicted to be localized inside the chloroplast with
40.4% probability. In Silico analysis of the protein sequences revealed the non-membranous nature
and might be played an important role in gene regulation. Global expression pattern analysis
revealed found to be highly expressed in the flowering stage.