2D & 3D STRUCTURE prediction of serum paraoxonase- III BY COMPARATIVE MODELLING
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Date
2010
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Abstract
This thesis elucidates the attempt of secondary structure and tertiary structure
prediction of the PON III protein by Comparative Modelling, which minimises the risk of
“Atherosclerosis” belongs to Serum Paraoxonase family.As the three dimensional structure
of PON-III, Swiss Prot Acc No. Q15166 has not yet been reported in the PDB hence it was
taken as target and then subjected to protein secondary structure prediction using SOPMA
and GORIV. It was found approximately 50 % of secondary structural elements in both cases
of results. From blast search results, the protein IV04Apdb found 60% of similarity with the
target hence taken as template. Using the template structure ten different models were
generated by Modeller9v8.The best model was chosen with highest verify 3D score. This
work would be further extended for conformational structure determination of the PON-III in
wet lab and better understanding the role of PON-III in “Atherosclerosis”.
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