Characterization of mango (Mangifera indica L.) hybrids using SSR markers
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Date
2011
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IARI, DIVISION OF FRUITS AND HORTICULTURAL TECHNOLOGY
Abstract
Mango (Mangifera indica L.) is the choicest fruit of India and occupies a prominent place among
the best fruits of the world. India is the leading producer of mango in the world. India is the
world’s richest mango germplasm centre and reportedly, there are over 1,000 varieties of mango
found in India. However, there is a lot of confusion in nomenclature of the mango cultivars.
Accurate characterization of the available germplasm is a prerequisite for their conservation as
well as utilization in the future breeding programmes. Traditionally mango genotypes have been
characterized using morphological markers. Morphological markers are limited in number, have
complex inheritance pattern, and are affected by environmental conditions. Contrary to this,
molecular markers are practically unlimited in number, remain unaffected by the environment
and growth conditions, and are simply inherited. Keeping in view this fact, the present
experiment was carried out for estimating the genetic diversity among mango hybrids and to
prepare their DNA fingerpints using SSR markers. Forty eight mango hybrids were analyzed
using 17 SSR markers. These primers detected 59 scorable loci, of which 45 were polymorphic.
The size of the alleles detected ranged from 100 bp to 480 bp. Polymorphis Information Content
(PIC) of SSR markers ranged from 0.023 to 0.295. The genetic relationship among mango
hybrids, based on Jaccard’s Similarity Coefficient, ranged from 0.38 to 0.97. The dendrogram,
based on UPGMA cluster analysis, grouped the mango hybrids into three major groups. The
Principal Coordinates Analysis also exhibited more or less similar distribution of mango hybrids.
Cultivar specific fingerprints were developed for the hybrids and represented as barcode
diagram. The findings of this study will prove helpful in the accurate identification of mango
hybrids facilitating germplasm conservation. It is expected that mango breeders will fruitfully
utilize the results of this study to expediate mango improvement programme in future.
Description
T-8552