Monitoring the Expressions of selected drought responsive Transcription factor genes in sorghum
Loading...
Date
2010
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UAS, Dharwad
Abstract
Five transcription factor (TF) families, the HAP2, MADS, ARF, HB and MBF
comprising 20 members were tested for their up-and down regulation in well watered and
water stressed shoot and root tissues of sorghum cv. M35-1. Selected genes were in-situ
hybridized in both tissue types as a function of moisture stress regime. The PTSb00029.1 and
PTSb00033.1 of ARF family and PTSn00174.1 and PTSb00175.1 of HB family recorded 2 to
5, PTSb00221.1 and PTSb00208.1 of MADS family and PTSb00128.1 of HAP2 family
recorded 5 to 10 fold up-regulation under moisture stress regimes. The PTSb00128.1, a
HAP2 family member, recorded 15 fold up regulation in mild stressed root tissues. TF genes
such as PTSb00218.1, PTSb00220.1, PTSb00031.1, PTSb00032.1, PTSb00034.1 and
PTSb00223.1 were found down regulating in both tissues types under stress condition.
However, the PTSb00128.1, PTSb00221.1, PTSb00029.1, PTSb00033.1 and PTSb00174.1
TFs were found up regulating to varied levels in mild and serve stressed root tissues only.
Taken together, moisture stress triggered up regulation of more genes in root tissue compared
to shoot tissue in sorghum.
Three TF genes, PTSb00220.1 (MADS), PTSb00223.1 (MBF) and PTSb00328.1
(WRKY) were selected based on their expression levels in qRT-PCR data and the DIGlabeled
ribo-probes along with 18S rRNA were prepared through in-vitro transcription for insitu
hybridization. Critical factors such as probe and buffer concentration and post
hybridization washing time were optimized using 18S rRNA probe in root and shoot tissues.
The in-situ expression pattern of these genes was studied at cellular level in well watered and
drought stressed shoot and root tissues. The expression could be localized to specific tissue
types and overall expression pattern all the three genes was comparable to pattern revealed in
qRT-PCR method. The expression of 18S rRNA was found to be constitutive in both tissue
types and stress regimes.
Description
Keywords
Plant Biotechnology