Detection of Subclinical Canine Ehrlichiosis by Nested PCR

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Date
2009-05
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Indian Veterinary Journal
Abstract
Whole blood samples from 80 dogs suspected of ehrlichiosis with fever and tick infestation were subjected to blood smear examination and Nested PCR (NPCR) for detection of Ehrlichia canis. Of these, NPCR revealed 47..5 per cent (38 out of 80) positive cases as against 1.25 per cent (one out of 80) by blood smear examination. As NPCR is a highly sensitive assay, it could be employed for diagnosis of early and subclinical infection of E. canis.
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