MICROBIAL CHARACTERIZATION OF ROOT KNOT NEMATODE (Meloidogyne incognita) INFECTED RHIZOSPHERE
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Date
2023
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Abstract
Present study was carried out to characterize the microbes associated with rootknot
nematode infected rhizosphere and also to evaluate their biocontrol potential against
two important nematode pests i.e., Meloidogyne incognita and M.graminicola in laboratory
condition. A total of 40 numbers of bacteria/fungal/actinobacteria were isolated from
nematode infested rhizospheric soil collected from Golghat, Nagaon and Majuli districts of
Assam. The isolates ANKBF1 and AMRCF2 were identified as Penicilium cetrinum and
Aspergillus terreus respectively and isolate AGKOB1 was identified as Bacillus pumilus
on the basis of molecular characterization. To evaluate their biocontrol potential, culture
filtrates of all the isolated bioagents were tested for egg hatching inhibition and second
stage juvenile mortality of root-knot nematode and rice root-knot nematode under
laboratory condition. Studies revealed that all the isolates of bacterial, fungal and
actinobacterial exhibited maximum egg hatching and juvenile mortality of root-knot
nematode (M. incognita and M. graminicola) in 25, 50 and 75 percent concentration of
filtrates at 12, 24, 48 and 72 hours of exposure under in-vitro condition. However, the
efficacy was varied among the isolates. Among the identified isolates the maximum egg
hatch inhibition (54%-61.17%) and the nematode (M. incognita and M. graminicola)
juvenile mortality (92.67%-96.33) with low LC50value (20.71%-13.01%) was observed in
75% concentration of fungal isolate ANKBF1 viz, Penicilium cetrinum as well as bacterial
isolates AMRCF2 viz, Bacillus pumilus at 72 hrs of exposures. This was followed by
isolate AMRCF2 ( Aspergillus terreus) prevent egg hatching range from (49.17%-50.50%)
at high concentration @ 75 percent and caused 73.67%-82% J2 mortality of root knot
nematodes after 72 hrs with LC50 value 25.12%-31.78%.