Molecular characterization for targeting foliar blast disease in pearl millet [Pennisetum glaucum (L.) R. Br.]
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Date
2023-04
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CCSHAU
Abstract
In the present investigation, 60 pearl millet genotypes were evaluated at experimental field of
Bajra Section, CCS HAU, Hisar during kharif season, 2021 for morpho-phenological traits &
blast disease and the study of genetic relatedness among them using microsatellite markers.
Highly significant mean sum of squares were obtained due to the genotypes for all the traits
studied. The traits viz., plant height, ear length, ear diameter, leaf blade width, effective
number of tillers per plant, 1000-grain weight, dry fodder yield per plant and grain yield per
plant exhibited high heritability (>60%) and high genetic advance as percent of mean
(GA%M) (>20%) whereas, the traits ear length, leaf blade width, effective number of tillers
per plant, dry fodder yield per plant and grain yield per plant exhibited high Genotypic
Coefficient of Variation (GCV) (>20%) and Phenotypic Coefficient of Variation (PCV)
(>20%). Correlation coefficient analysis revealed high significant positive correlation
coefficients of grain yield with traits viz., plant height, ear diameter and dry fodder yield per
plant. As per blast screening, 43 genotypes were found resistant to the disease while 17
genotypes were found susceptible. In order to develop blast resistant hybrids, selected
resistant lines were crossed with elite lines of pearl millet and out of the 34 hybrids
developed, 32 were found resistant to blast. The increased activities of Phenylalanine
Ammonia Lyase (PAL), Peroxidase (POX) and Lipoxygenase (LOX) in the resistant hybrids
further confirmed the disease scoring results