Molecular characterization of indigenous chicken breeds using RAPD-PCR
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Date
2023-03-27
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Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu (J&K)
Abstract
The present study was carried out on a total of hundred chicken including 50 each of Kadaknath and Aseel chicken breeds with the objectives to study genetic variation between two breeds of chicken using RAPD-PCR technique, to establish genetic relationship and genetic distance between these breeds. The blood samples of Kadaknath and Aseel chicken were collected from local farms of RS Pura, Jammu. DNA was isolated by HiPurA Blood Genomic DNA Miniprep Purification Kit (Hi-media). Six primers were screened for RAPD study of two chicken breeds. But, only four primers generated reproducible and distinct RAPD profiles. A total of 15 loci were found in Kadaknath and Aseel chicken by four different RAPD primers out of which 9 were polymorphic. Average no. of bands ranged from 1.66 to 4.94. The mean effective number (ne) of allele for Kadaknath was 1.5886 ± 0.4977 and Nei’s gene diversity (h) was 0.2971 ± 0.2511. The Shannon’s information index (I) was 0.4130 ± 0.3491 in Kadaknath. The mean effective number (ne) of allele for Aseel was 1.5642 ± 0.4809 and Nei’s gene diversity (h) was 0.2902 ± 0.2459. The Shannon’s information index (I) was 0.4060 ± 0.3436 in Aseel. The genetic identity between Kadaknath and Aseel breeds was high and it was 0.9886. On the other hand the genetic distance between Kadaknath and Aseel was 0.0114. The genetic diversity reported among chicken breeds should be expanded by exploring more advanced analysis of genetic markers.
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