Evaluation of Therapeutic Potential of Bleomycin and Catechin Combination in Animal Model of Mammary Tumor
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Date
2023-04-03
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MAFSU, Nagpur
Abstract
Cancer is the second leading cause of fatality all over the world. Various unwanted side effects are being reported with the use of conventional chemotherapy. The plant derived bioactive compounds are the prominent alternative medicinal approach for reduction of chemotherapy associated side effects. Catechins are significant components of tea leaves and have powerful antioxidant and physiological effects.
This study was carried out to evaluate the therapeutic potential of bleomycin and catechin combination in animal model of mammary tumour. In the present study, a total of 40 female Wistar rats (08 normal; 32 DMBA treated rats) were used and divided into five groups comprising of eight animals in each group namely group A (normal control), group B (positive or disease control), group C (treatment with bleomycin), group D (treatment with catechin) and group E (treatment with both catechin and bleomycin).
The tumours were induced by DMBA in female Wistar rats via intragastric tube. After 45-60 days, the animals from Group B, C, D and E were showing the clinical signs of development of tumorous tissue in the abdominal and peripheral skin tissue and on the back of animals. The body weights were decreased in tumour induced groups on day 60 and on day 90 as compared to normal control group and treatment group.
Among haematological parameters TLC was significantly improved in treatment groups. The serum biochemical parameters, serum estrogen levels and histopathological lesions showed significant improvement after the treatment of catechin combination with bleomycin
Thus, the present data strongly suggests that the catechin has anti-tumour potentials and concludes that the mechanistic study of catechin in combination with bleomycin can further be conducted as dose rate reduction of bleomycin was significantly proved in present study in DMBA induced rats.