EFFECT OF EFFIECIENT NATIVE ISOLATES OF RHIZOBIUM ON GROWTH AND BIOMASS OF FIELDBEAN (Dolichos lablab L.) UNDER POT CULTURE
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Date
2023-12-29
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Abstract
Fieldbean (Dolichos lablab L.) belongs to the family fabaceae, is a pulse-cum
vegetable crop of India cultivated for its tender and mature pods, seeds and fodder. Pot
culture Experiment was conducted in Green house condition to study the effect of efficient
native isolates of Rhizobium on growth and Biomass of fieldbean. (Dolichos lablab L.)
Rhizobium isolates were isolated from the different root nodules of legume crops of kalyana
karanataka region. Characterized by standard biochemical tests.
All Rhizobium isolates were found to be Gram negative and short rods, smooth,
raised, circular, yellow, white, semi translucent and mucilaginous colonies on Yeast Extract
Mannitol Agar Medium (YEMA) after 3-4 days of incubation, they were acid producers and
fast growers. Isolates produced white to light pink colonies indicating that they did not absorb
or weakly absorb the congored colour on Yeast Extract Mannitol Agar with Congo Red
(CRYEMA). Isolates showed negative for Glucose peptone agar, Ketolactose, Hofers
Alkaline Broth, lactose sugar and Starch hydrolysis and positive for Catalase and Acid from
glucose test.
All the isolates were further screened for their plant growth promoting properties such
as in vitro nitrogen fixation, P solubilisation, IAA, and siderophore production. Combined
inoculation of native efficient isolates (RRR-4, RRC-11 and RRC-15) of Rhizobium of field
bean significantly enhanced growth and biomass with respect to plant height (63.20 cm),
number of leaves (14.04), number of branches (8.32), number of nodules (19.65), Dry matter
accumulation (22.65 g), number of pods (21.60), number of seeds (3.81), and % of plant
nitrogen uptake (0.374 %) over uninoculated control. These isolates proved to be best
campare with earlier isolates can be used to prepare bioinoculants of legume crops of this
region along with reduced RDF. And found to be superior