Characterization of Fusarium species associated with pokkah boeng disease of sugarcane
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Date
2023
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Punjab Agricultural University
Abstract
Sugarcane (Saccharum spp. hybrid complex) is an important cash crop of India. Among the various fungal diseases, pokkah boeng caused by Fusarium spp. has emerged as an important disease in this crop. As the occurrence of this disease has been reported from Punjab till date the cultural, morphological and molecular aspects of this disease are not studied. Keeping in view, the present research was carried to characterize the Fusarium spp. associated with pokkah boeng disease of sugarcane. Survey was conducted in the sugarcane growing command area of sugar mills (nine co-operative and seven private) of Punjab during the year 2021-22. The maximum average incidence of pokkah boeng was reported on commercially grown variety Co 0238 (17.70 %) which depicted that the said variety is highly susceptible for pokkah boeng disease. Thirty isolates of Fusarium were obtained after isolation and purification from diseased samples collected during the survey. On the basis of pathogenicity tests, all the test isolates of Fusarium found to be virulent by producing necrotic lesion in detached leaf assay and typical symptoms of pokkah boeng in syringe method. Among all the isolates, Fs23 and Fs25 were highly pathogenic. The isolates showed significant variations on basis of their cultural and morphological characteristics and were categorized into various groups. Molecular study of isolates revealed that isolates Fs7, Fs20, Fs27, Fs29 showed 100 per cent similarity with the ITS, RPB2 and TEF-1α gene sequences of F. sacchari available in GenBank whereas isolates Fs13 and Fs17 showed 99 per cent similarity with F. proliferatum. Hence, the two species of Fusarium namely F. sacchari and F. proliferatum were identified as causal pathogens associated with pokkah boeng disease of sugarcane in Punjab. Genetic diversity estimate by seven ISSR primers showed high degree of polymorphism. Two isolates Fs16 (Jalandhar) and Fs18 (SBS Nagar) were found to be highly diverse among all test isolates. Sugarcane clones along with commercially released varieties were screened against pokkah boeng to identify the resistance sources in which 23, 18 and four clones/varieties showed resistant, moderately resistant and moderately susceptible disease reaction, respectively.
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Meena, Manisha (2023). Characterization of Fusarium species associated with pokkah boeng disease of sugarcane (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.